Reaction Vessel Stirring Mechanism for Plasmonic Detection Accuracy
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Solution Overview
Problem
Existing detection methods, such as those described in JP 2007-064943 A, face challenges in maintaining measurement accuracy and reproducibility due to decreased substance concentration near the capturing region and difficulties in adjusting reaction time, especially when dealing with small amounts of specimens.
Innovation Solution
A detection method and device utilizing a reaction vessel with a stirring mechanism to ensure contact between the liquid and the capturing region only during stirring, allowing for high accuracy and reproducibility by controlling the liquid amount and position relative to the capturing region.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If the liquid containing the substance to be detected is retained in the flow path, then the binding reaction can proceed, but the concentration of the substance to be detected in the vicinity of the capturing region decreases, reducing measurement accuracy
Solution Approach 1:
The invention introduces a stirring mechanism that dynamically moves the liquid containing the substance to be detected toward the capturing region. This dynamic approach ensures continuous replenishment of the substance concentration at the capturing region, preventing the concentration decrease that occurs with static liquid retention, thereby maintaining high measurement accuracy.
2Measurement precision
If the liquid containing the substance to be detected continues to flow in the flow path to maintain measurement accuracy, then a large amount of liquid is required
Solution Approach 1:
The invention extracts the liquid from the flow path and introduces it into the reaction chamber where stirring occurs. This separation allows the liquid to be contained in a controlled environment with a smaller volume, while the stirring mechanism ensures adequate mixing and contact with the capturing region, thus maintaining measurement accuracy without requiring large amounts of liquid.
3Duration of action of moving object
If the liquid containing the substance to be detected is continuously flowed to ensure binding reaction saturation, then the reaction time can be controlled, but the amount of liquid required increases
Solution Approach 1:
The invention employs periodic stirring action in the reaction chamber to achieve binding reaction saturation. The stirring mechanism periodically moves the liquid toward the capturing region, ensuring continuous contact and reaction without requiring continuous liquid flow. This periodic action allows precise control of reaction time while minimizing the total amount of liquid needed.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables the detection of substances with high accuracy and reproducibility even when the specimen amount is small, by efficiently capturing and binding the substance to the capturing region through controlled stirring and liquid management.
Implementation Method 1
irradiating the metal film with light so that surface plasmon resonance occurs in the metal film
Data Source
AI summary
A detection method uses a reaction vessel including a container including a housing part having a first opening opened at an upper portion and a second opening opened at a side portion, and a side wall member fixed to the container so that a capturing region on a metal film is exposed in the second opening. First, a liquid containing a specimen is provided to the housing part. Next, the liquid in the housing part is stirred to capture, into the capturing region, a substance to be detected in the liquid. Next, the metal film is irradiated with light so that surface plasmon resonance occurs in the metal film, and light emitted from the reaction vessel and having a light amount changing depending on the amount of the substance to be detected captured in the capturing region is detected. In the step of providing the liquid to the housing part, an amount of liquid in which the liquid is not in contact with the capturing region when the liquid in the housing part is left still, and the liquid is in contact with the capturing region when the liquid in the housing part is stirred is provided to the housing part.