Reagent Preparing Device Calibration for Flow Cytometry
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Solution Overview
Problem
Existing reagent preparing devices for flow cytometry may supply reagents of low quality due to inaccuracies in electrical conductivity measurements, leading to unreliable analysis results, as they rely solely on concentration sensors without proper calibration and quality control.
Innovation Solution
A reagent preparing device equipped with a characteristic measurement device and a controller that measures and calibrates the reagent's properties based on known standards, ensuring accurate concentration and quality of the reagent supplied to the measurement section.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Extent of automation
If electrical conductivity measurement is used to determine reagent concentration, then reagent preparation can be automated, but measurement accuracy deteriorates due to sensor deviation from true values
Solution Approach 1:
The system performs preliminary calibration by measuring the electrical conductivity of a standard liquid with known concentration before actual reagent preparation. This preliminary measurement establishes the relationship between conductivity and concentration, creating a reference that compensates for sensor deviations and ensures accurate concentration determination during automated operation
Solution Approach 2:
The system implements feedback control by comparing the measured electrical conductivity against the calibrated relationship and adjusting the reagent preparation process accordingly. The controller uses the calibration data to continuously monitor and correct concentration deviations, ensuring accurate reagent preparation throughout automated operation
2Reliability
If reagent supply is controlled based on concentration sensor readings, then reagent quality can be maintained, but unreliable measurements lead to supply of low-quality reagent
Solution Approach 1:
The system performs preliminary calibration using a standard liquid with known concentration characteristics before supplying reagent to the measurement section. This calibration establishes an accurate reference relationship that enables reliable quality control throughout the reagent supply process, preventing low-quality reagent from being supplied
Solution Approach 2:
The controller uses feedback from the characteristic measurement device to monitor reagent concentration and adjust supply decisions. By comparing real-time measurements against the calibrated reference, the system ensures only reagent meeting quality specifications is supplied to the measurement section
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This solution ensures the supply of reagents with consistent and accurate concentrations, enhancing the reliability of analysis results by continuously monitoring and calibrating the reagent's properties, thereby preventing low-quality reagent supply.
Implementation Method 1
a concentration sensor including an electrical conductivity meter is arranged in the preparing tank
Data Source
AI summary
A reagent preparing device capable of supplying a predetermined reagent, which includes a first liquid and a second liquid different from the first liquid, to a measurement section for measuring a specimen using the reagent, comprising: a reagent preparing section for preparing the predetermined reagent; a characteristic measurement device for measuring characteristic of the reagent prepared by the reagent preparing section; and a controller configured for performing operations comprising, controlling the supply of reagent prepared by the reagent preparing section to the measurement section according to the measurement result by the characteristic measurement device, and calibrating the characteristic measurement device based on a known characteristic value of a standard liquid having the known characteristic value and a measurement result obtained by measuring the characteristic of the standard liquid by the characteristic measurement device, is disclosed. A specimen processing system is also disclosed.