High Rebaudioside M Stevia Plant Breeding

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Solution Overview

Problem

Rebaudioside M, known for its good taste quality among steviol glycosides, cannot be obtained in large amounts from natural stevia plants, making its obtainment a concern.

Innovation Solution

A high rebaudioside M content non-genetically modified stevia plant is developed, containing 2% or more of rebaudioside M with respect to the amount of total steviol glycoside in a leaf, along with a method for producing and screening such plants.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Quantity of substance

If conventional stevia plants are used, then other steviol glycosides (such as rebaudioside A) can be obtained, but rebaudioside M cannot be obtained in large amounts

Engineering Contradiction:

Improverebaudioside M contentVSAvoidobtainment efficiency of rebaudioside M

Core Design Contradiction:

Quantity of substanceVSProductivity

Solution Approach 1:

The patent applies parameter changes by identifying specific genetic markers (SNP loci) associated with high rebaudioside M content and using these markers to select and breed plants with desired traits. This changes the genetic parameters of the stevia plant to achieve higher rebaudioside M production without genetic modification

Inventive Principle:

Principle #35Parameter changes

Solution Approach 2:

The patent performs preliminary action by developing and validating DNA markers before breeding programs. The markers are identified and characterized in advance, allowing for early selection of plants with high rebaudioside M potential, thus streamlining the breeding process and improving obtainment efficiency

Inventive Principle:

Principle #10Preliminary action

2Quantity of substance

If natural stevia plants are used, then no genetic modification is needed, but rebaudioside M content is too low for commercial viability

Engineering Contradiction:

Improverebaudioside M contentVSAvoidcommercial production feasibility

Core Design Contradiction:

Quantity of substanceVSEase of manufacture

Solution Approach 1:

The patent applies self-service by using the plant's own genetic information (DNA markers) to identify and select high-producing variants. The natural genetic variation within stevia populations is harnessed through marker-assisted selection, allowing the breeding program to efficiently identify plants with desirable traits without external genetic modification

Inventive Principle:

Principle #25Self-service

Solution Approach 2:

The patent implements feedback by using DNA marker analysis to monitor and select plants with the desired genetic profile for high rebaudioside M content. The marker data provides feedback on the genetic composition of breeding stock, enabling informed selection decisions that progressively improve rebaudioside M content in subsequent generations

Inventive Principle:

Principle #23Feedback

Data Source

PatentUS12317798B2Rebaudioside M-rich stevia plant

Publication Date: 2025.06.03 SUNTORY HLDG LTD

AI summary

The present invention provides a high rebaudioside M content non-genetically modified stevia plant comprising rebaudioside M at higher content as compared with the wild type stevia species. The present invention also provides a method of producing such a high rebaudioside M content non-genetically modified stevia plant, and a dry leaf obtainable from such a plant.