Receptor-Binding Disabled Polypeptides for Pest Resistance Management
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Existing insecticidal proteins used in crop protection face challenges in specificity and persistence, leading to non-target effects and the rapid development of resistance in pest populations, necessitating a method for identifying toxins with distinct modes of action that do not compete for the same receptor.
Innovation Solution
The development of disabled polypeptides derived from fully functional pesticidal proteins, which retain receptor binding but reduce toxicity, combined with fully functional proteins to create compositions that inhibit pest infestation while minimizing resistance development.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If a single insecticidal protein is used to control target pests, then pest control efficacy is achieved, but resistance develops rapidly in pest populations
Solution Approach 1:
The patent combines multiple insecticidal proteins with different modes of action into a single composition or plant expression system. This merging approach maintains effective pest control while extending the duration of resistance management by targeting multiple distinct receptors simultaneously, thereby preventing rapid resistance development that occurs with single-protein applications.
Solution Approach 2:
The invention creates a multi-functional insecticidal system where a single composition or transgenic plant expresses multiple toxin proteins that act through different mechanisms. This universality allows the system to control pests through multiple pathways, reducing the likelihood that a single resistance mechanism will render the entire system ineffective.
2Reliability
If insecticidal proteins are applied extensively to control pests, then pest infestation is reduced, but non-target effects and environmental persistence issues occur
Solution Approach 1:
The patent employs insecticidal proteins with highly specific local qualities, where each toxin protein is designed to bind to specific insect receptors with high affinity and selectivity. This specificity ensures that the proteins affect only their target pest species while leaving non-target organisms unharmed, thereby reducing non-target effects while maintaining effective pest control.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach ensures effective pest control with reduced likelihood of resistance by using toxins that bind to different receptors, maintaining efficacy and reducing the onset of resistance in target pests.
Implementation Method 1
disabled polypeptides derived from fully functional pesticidal proteins, which retain receptor binding but reduce toxicity
Implementation Method 2
the DP, when used in a plurality of molar ratios with a constant amount of a second FFPP, does not titer the toxic properties of the second FFPP
Data Source
AI summary
Pesticidal proteins (FFPP's) are used to produce derivatives (DP's) that are ineffective and disabled relative to conferring toxic properties upon a target pest, yet the ability of the DP to bind to the receptor to which said FFPP binds is unaffected. Such DP's are useful in inhibiting the FFPP from which it was derived when both are fed to a target pest and for comparing receptor binding capability and efficiency relative to different FFPP's from which the DP has been derived, providing for an assessment of different FFPP's relative to each other, and providing uniformity and certainty in combinations of such FFPP's for compositions, including transgenic plants, that can be used to control pest populations susceptible to both FFPP's, creating more durable transgenic plant products, inhibiting the development of resistance to such FFPP's when used in plants commercially, and in providing a durable and viable resistance management strategy for crops using such FFPP combinations. Polynucleotide sequences intended for use in expression of the DP's and FFPP's are also provided. Particular embodiments provide methods of designing and preparing DP's, as well as compositions and methods of using DP's and the FFPP's from which the DP's have been derived in more effective pesticidal compositions and products.