Reciprocating Cell Concentration via Pneumatic Pushing
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Solution Overview
Problem
Traditional cell concentration and purification technologies, such as centrifugation and peristaltic pumps, often cause shearing force and damage to fragile cells, leading to inefficient and inaccurate concentration processes.
Innovation Solution
A reciprocating concentration system that includes a gas output device, liquid accommodating tanks, selection devices, sensors, and a computing control device, which uses pushing gas to reciprocally pass liquid through a selection device, reducing shearing force and enhancing filtration efficiency.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If traditional centrifugation or peristaltic pumps are used for cell concentration, then concentration efficiency is improved, but shearing force and damage to fragile cells increase
Solution Approach 1:
The patent uses gas pressure to push liquid through the selection device, replacing mechanical pumps that generate shearing forces. The gas output device applies pressure to the liquid in the liquid accommodating tank, causing gentle flow through the filtration membrane without mechanical contact or high shear stress, thus protecting fragile cells while maintaining concentration efficiency
Solution Approach 2:
The invention replaces the mechanical peristaltic pump system with a pneumatic system. Instead of using mechanical rollers to compress and move liquid through tubing (which creates shear stress), the system uses gas pressure to drive liquid flow, eliminating the harmful mechanical shearing forces while achieving the same liquid transport and concentration function
2Manufacturing precision
If higher concentration accuracy is achieved, then purification quality is improved, but processing time increases
Solution Approach 1:
The patent implements continuous reciprocating flow through the selection device, where liquid is continuously pushed back and forth through the filtration membrane. This continuous action maintains high concentration accuracy by ensuring complete filtration while preventing stagnation, achieving both high purification quality and efficient processing time
Solution Approach 2:
The system uses periodic reciprocating motion to push liquid through the selection device and back. The gas output device alternates applying pressure to move liquid in one direction, then releases to allow backflow, creating a rhythmic filtration process that maintains high concentration accuracy while optimizing processing speed through continuous cyclic operation
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The system effectively reduces shearing force and damage to cells, achieving higher cell viability (>90%) and concentration accuracy (≤5%), while improving the efficiency of cell extraction and purification.
Implementation Method 1
a gas output device (11), a first liquid accommodating tank (12), a second liquid accommodating tank (13)... The gas output device is controlled to output pushing gas through a first gas channel (101) or a second gas channel (102)
Data Source
AI summary
A reciprocating concentration system includes: a gas output device, first and second liquid accommodating tanks, a selection device, first and second liquid sensors and a computing control device. The gas output device is controlled to output pushing gas through a first gas channel or a second gas channel. The first liquid accommodating tank is in communication with the first gas channel. The second liquid accommodating tank is in communication with the second gas channel. The selection device is in communication with the first liquid accommodating tank and the second liquid accommodating tank through a first liquid channel and a second liquid channel, respectively, and has a filtered liquid outlet. The first liquid sensor and the second liquid sensor are disposed at the first liquid channel and the second liquid channel, respectively. The computing control device is connected to the gas output device and the first and the second liquid sensors.