Recombinant Viral Antigen Display for Safe Field Virus Detection
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Solution Overview
Problem
Current methods for detecting dangerous viruses require level 3 biocontainment facilities and sophisticated equipment, are time-consuming, and not suitable for field use, posing risks to operators and delaying timely treatment and quarantine.
Innovation Solution
Development of recombinant viruses and insect cells expressing viral proteins on their surface, which can be used as antigens for rapid and safe detection of viral infections without the need for specialized facilities or equipment.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If traditional virus cultivation and isolation methods are used, then accurate virus identification can be achieved, but it requires level 3 biocontainment facilities and sophisticated equipment, increasing device complexity and operational difficulty
Solution Approach 1:
The patent extracts the viral protein antigen from the complete virus structure, allowing detection without handling the entire dangerous virus. Only the essential antigenic component is isolated and displayed on a safe carrier virus or insect cell, eliminating the need for high-level biocontainment facilities while maintaining diagnostic accuracy
Solution Approach 2:
The patent uses a recombinant carrier virus or insect cell as an intermediary to display the viral antigen. This intermediary allows the antigen to be presented in a safe, controlled manner without requiring direct handling of the dangerous pathogen, thus reducing facility requirements while preserving detection capability
2Measurement precision
If traditional virus cultivation and DNA sequencing methods are used, then virus identity can be confirmed, but the process is time-consuming, increasing detection time
Solution Approach 1:
The patent performs preliminary action by pre-expressing the viral antigen on the carrier virus or insect cell before detection. This eliminates the need for time-consuming virus cultivation and DNA sequencing steps during actual detection, as the antigen is already available in a detectable form, significantly reducing detection time while maintaining accuracy
Solution Approach 2:
The patent replaces the complex mechanical and procedural system of virus cultivation and DNA sequencing with a simplified antigen-antibody binding assay. This substitution eliminates multiple time-consuming steps while achieving the same diagnostic goal through a more direct immunological detection method
3Ease of operation
If purified viral proteins are used as antigens, then detection can be performed, but production is time-consuming and labor-intensive, decreasing productivity
Solution Approach 1:
The patent enables self-service by using the recombinant carrier virus or insect cell system to automatically produce and present the viral antigen. The system self-generates the detectable antigen without requiring external purification steps, making the antigen production integrated into the detection platform itself and eliminating labor-intensive processing
Solution Approach 2:
The patent merges the antigen production and detection functions into a single integrated system. The recombinant carrier virus or insect cell both produces the antigen and serves as the detection platform, combining multiple steps into one unified approach that improves productivity while maintaining ease of operation
4Measurement precision
If traditional detection methods are used, then accurate diagnosis can be achieved, but operators are exposed to dangerous pathogens, decreasing safety
Solution Approach 1:
The patent extracts only the essential antigenic component from the dangerous virus and displays it on a safe carrier. This allows accurate diagnosis to be achieved without operators being exposed to the complete pathogenic virus, as the carrier virus or insect cell does not retain the dangerous properties of the original pathogen
Solution Approach 2:
The patent converts the harmful pathogen into a beneficial diagnostic tool by using its antigenic components displayed on a safe carrier. The dangerous viral proteins are repurposed for detection purposes while the pathogenicity is eliminated, transforming a harmful element into a safe and useful diagnostic reagent
Data Source
AI summary
Provided herein are recombinant viruses and/or insect cells suitable for detecting the infection of a pathogen in a biological sample of a test subject. The information derived from the detection may also be used to render a diagnosis on whether the test subject is infected with the pathogen or not, so that proper course of treatment may be assigned to the subject. Also provided herein is a vaccine for the prophylaxis and/or treatment of infection caused by the pathogen.