Recombinant Cell Surface Protein Control via Oligonucleotide Hybridization
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Solution Overview
Problem
Current systems lack the ability to easily attach, control, and reverse the attachment of proteins on cell membranes, limiting control over cellular processes and adaptability to environmental changes.
Innovation Solution
A system comprising recombinant cells expressing polypeptides with membranal anchoring and extracellular binding domains, combined with oligonucleotide-bound compounds that hybridize to attach and detach synthetic agents, allowing for dynamic modification of cell surface proteins.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If cell surface proteins are used to mediate cellular processes, then cellular functions are achieved, but the ability to dynamically control and reverse protein attachment is limited
Solution Approach 1:
The patent introduces oligonucleotide-based intermediaries that mediate the attachment and detachment of synthetic agents to cell surface proteins. These intermediaries hybridize to the proteins, enabling controlled attachment and reversible detachment through complementary strand binding, thus providing dynamic control over protein attachment without permanently altering cellular functions
Solution Approach 2:
The system enables dynamic modification of cell surface proteins by allowing attachment and detachment of synthetic agents in response to environmental cues. The reversible hybridization of oligonucleotides creates a dynamic system where protein composition can be changed and reversed, transforming static cell surface structures into dynamically controllable interfaces
2Adaptability or versatility
If synthetic agents are attached to cell surfaces, then control over cellular processes is improved, but the complexity of the system increases
Solution Approach 1:
The oligonucleotide-based system provides multi-functionality by enabling attachment of various synthetic agents (fluorescent dyes, drugs, antibodies) to different cell surface proteins through a universal hybridization mechanism. This universal approach simplifies the system compared to protein-specific attachment methods while maintaining the ability to control diverse cellular processes
Solution Approach 2:
The system controls cellular processes by changing parameters such as oligonucleotide sequence, concentration, and hybridization conditions rather than requiring complex mechanical or structural modifications. This parameter-based control approach simplifies system operation while achieving precise control over protein attachment and cellular responses
3Adaptability or versatility
If cell surface proteins are modified, then adaptability to environmental changes is improved, but perturbation of cellular functions may occur
Solution Approach 1:
The patent extracts the attachment function from the cell surface proteins themselves, using separate oligonucleotide intermediaries that bind to proteins without permanently altering them. This extraction allows synthetic agents to be attached and detached without modifying the intrinsic properties or stability of the cellular proteins, thus maintaining cellular function reliability while achieving adaptability
Solution Approach 2:
The system enables temporary attachment of synthetic agents that can be discarded or detached when no longer needed, while the cell surface proteins remain intact and recoverable. The reversible oligonucleotide hybridization allows agents to be removed without damaging the underlying proteins, maintaining cellular function stability while providing temporary adaptability for specific experimental or therapeutic needs
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables precise control over cell surface protein composition and interactions, facilitating adaptability and reversibility without perturbing cellular functions.
Implementation Method 1
a second compound comprising a second oligonucleotide (ODN-2) covalently bound to a synthetic agent, either directly or through a second linker, wherein said second oligonucleotide is complementary to said first oligonucleotide
Data Source
AI summary
The disclosure presented herein provides artificial receptors and a system comprising recombinant cells decorated with various labels and/or synthetic agents, wherein said labels and/or synthetic agents can be reversibly modified or removed from the cells. Also disclosed herein are methods for decorating and/or modifying the cells and methods for using thereof.