Recombinant Collagen Peptide Transdermal Absorption via Segmentation
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Solution Overview
Problem
Existing collagen products face challenges in achieving effective transdermal absorption due to collagen's large molecular weight and limited skin penetration.
Innovation Solution
A type I recombinant collagen is constructed using a short amino acid sequence derived from natural human type I collagen, specifically the sequence GAPGAPGSQGAPGLQGMPGER, repeated three or more times, to enhance transdermal absorption performance.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If collagen solution is impregnated in a solid carrier to prolong interaction time, then transdermal absorption is promoted, but device complexity and manufacturing difficulty increase
Solution Approach 1:
The collagen molecule is segmented into smaller peptide units (e.g., GPLGPG tripeptide repeats) that can be independently synthesized and then assembled. This segmentation allows the collagen to penetrate skin more effectively while maintaining the structural integrity and functional properties of the original collagen through controlled assembly of these smaller units.
Solution Approach 2:
The molecular weight parameter of collagen is changed by controlling the degree of polymerization of the repeating peptide units. By adjusting the number of repeats and the size of collagen aggregates, the patent optimizes the balance between transdermal penetration capability and structural stability, achieving effective absorption without requiring complex mask towel structures.
2Reliability
If large molecular weight collagen molecules are used to maintain structural integrity, then biological activity is preserved, but transdermal penetration ability deteriorates
Solution Approach 1:
Collagen is divided into repeating peptide units (such as GPLGPG tripeptides) that can independently penetrate the skin. These segmented units retain the essential biological activity of collagen while being small enough to cross the skin barrier, solving the contradiction between molecular size and penetration ability.
Solution Approach 2:
The patent employs a nested structure where smaller peptide units are assembled into larger collagen-like structures. The repeating GPLGPG motifs nest within each other to form helical structures that mimic natural collagen, allowing small penetrating units to self-assemble into larger functional structures after skin penetration.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The recombinant collagen demonstrates excellent transdermal absorption performance, with specific sequences showing significant penetration over 24 hours, as verified by in vitro diffusion cell experiments.
Implementation Method 1
the interaction time between the collagen solution and the skin surface can be prolonged and promotes the penetration of collagen molecules with large molecular weight
Data Source
AI summary
The present invention relates to a high-transdermal-absorption peptide and I-type recombinant collagen constructed by means of repetitions of the peptide. The high-transdermal-absorption I-type recombinant collagen of the present invention is formed by means of multiple repetitions of the twenty-first peptide amino acid sequence from natural human I-type collagen as a repeating unit, wherein the sequence of the twenty-first peptide amino acid is as shown in SEQ ID No.: 1, and the number of repetitions is at least three. The high-transdermal-absorption I-type recombinant collagen can be used as a raw material for producing various collagen products, such as injections, facial fillers, dressings, cosmetics, healthcare foods, tissue engineering materials, collagen sponges, etc.