Recombinant Mannanase Stability in Detergents and Feed
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Solution Overview
Problem
Mannans in industrial applications, such as laundry and animal feed, pose challenges due to their viscous nature and high water binding capacity, leading to issues like reduced digestibility and stability of mannan-degrading enzymes in varying conditions.
Innovation Solution
Development of an enzyme composition comprising mannanases with at least 80% sequence identity to SEQ ID NO: 16, produced in recombinant host cells like Trichoderma reesei or Bacillus, which exhibit stability and activity in detergents and diverse chemical environments, facilitating efficient mannan degradation and modification.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If mannanases are used in industrial applications (laundry, feed, food), then mannan degradation activity is improved, but enzyme stability in varying storage and use conditions deteriorates
Solution Approach 1:
The patent applies parameter changes by modifying the enzyme's amino acid sequence (specifically residues in the catalytic domain and glycosylated regions) to alter its stability properties while preserving mannan degradation activity. The sequence variations enable the enzyme to maintain functionality across different storage and use conditions.
Solution Approach 2:
The patent creates composite enzyme formulations combining multiple mannanase variants (Man6, Man7, Man14) with different functional characteristics. This composite approach allows the enzyme composition to achieve both high mannan degradation activity and improved stability through the synergistic effects of multiple enzyme variants.
2Quantity of substance
If mannan is used in high amounts in feed, then fiber intake is improved, but digestibility and animal growth rate deteriorate due to viscosity and water binding
Solution Approach 1:
The patent extracts and degrades the problematic portions of mannan (the polysaccharide structure causing viscosity and water binding) using mannanase enzymes. By breaking down the mannan polymer chains into smaller oligosaccharides, the enzyme removes the harmful viscous properties while preserving the beneficial fiber content.
Solution Approach 2:
The patent uses mannanase enzymes as intermediaries to facilitate the conversion of difficult-to-digest mannan into more digestible forms. The enzymes act as mediators that bridge the gap between high fiber intake and improved animal growth by enzymatically modifying the mannan structure.
3Ease of manufacture
If mannan is used in laundry applications, then staining and soil removal is improved, but enzyme stability in detergent conditions deteriorates
Solution Approach 1:
The patent modifies the enzyme's structural parameters (amino acid sequence, glycosylation patterns) to enhance its stability in detergent conditions. The sequence variations specifically target regions that interact with detergent components, allowing the enzyme to maintain both stain removal efficiency and stability in varying detergent formulations.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The enzyme composition ensures effective mannan degradation, improving feed digestibility, animal growth rates, and laundry performance while maintaining stability across different conditions, enhancing the nutritional value of feeds and the efficiency of industrial processes.
Implementation Method 1
Enzymatic degradation of mannans reduces digesta viscosity of high water soluble mannans and leads to production of manno-oligosaccharides
Implementation Method 2
The mannanases of the enzyme composition of the invention are suitable for degrading and modifying mannan containing material in various chemical environments
Data Source
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AI summary
The present description is related to novel mannanases, compositions comprising mannanase, to methods for producing mannanases and to methods of using mannanases to degrade and modify mannan containing material.