Recombinant Microorganism Medium Chain Diol Production
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Solution Overview
Problem
Current methods for producing medium chain diols using microorganisms face challenges due to safety concerns with pathogenic strains and low yields, as these microorganisms harbor both ω-oxidative and β-oxidative metabolism pathways, leading to intermediate product issues.
Innovation Solution
A recombinant microorganism is developed by deleting fatty alcohol dehydrogenase and oxidase genes in the ω-oxidative pathway, fatty aldehyde dehydrogenase gene, and β-oxidative metabolism pathway-related genes, allowing for high-yield production of medium chain diols from fatty acid-derived alcohols or alkanes.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If microorganisms with both ω-oxidative and β-oxidative metabolism pathways are used for medium chain diol production, then the microorganisms can metabolize fatty acids through multiple pathways, but the medium chain diol yield is low due to competing β-oxidation consuming the intermediate products
Solution Approach 1:
The patent removes the β-oxidative metabolism pathway from the microorganism by deleting specific genes (acpP, acs, acyl-CoA oxidase genes). This extraction eliminates the competing metabolic route that consumes medium chain diol intermediates, thereby resolving the contradiction by maintaining only the ω-oxidative pathway for high-yield diol production
2Productivity
If pathogenic microorganisms are used for medium chain diol production, then high productivity can be achieved, but safety concerns arise due to the pathogenic nature of these microorganisms
Solution Approach 1:
The patent uses non-pathogenic microorganisms (such as engineered E. coli or yeast) and converts the previously harmful β-oxidative pathway into a beneficial configuration by deleting it. This allows achieving high productivity through optimized ω-oxidation while eliminating safety risks associated with pathogenic strains
3Productivity
If fatty alcohol dehydrogenase and oxidase genes are present in the ω-oxidative pathway, then complete oxidation to carboxylic acids occurs, but medium chain diol accumulation is prevented
Solution Approach 1:
The patent performs preliminary action by deleting the fatty alcohol dehydrogenase (fadH) and oxidase (oxo) genes before the oxidation process occurs. This prevents the conversion of fatty alcohols to aldehydes and subsequently to carboxylic acids, thereby allowing medium chain diols to accumulate as the primary oxidation products
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The recombinant microorganism effectively prevents further oxidation and β-oxidative metabolism, enabling high-yield production of medium chain diols like 1,12-dodecanediol, enhancing safety and industrial applicability.
Implementation Method 1
fatty alcohol dehydrogenase and oxidase genes in an ω-oxidative metabolism pathway are deleted
Implementation Method 2
β-oxidative metabolism pathway-related genes are also deleted
Data Source
AI summary
The present invention relates to a method for producing medium chain diol and, more particularly to recombinant microorganisms in which fatty alcohol dehydrogenase and/or fatty alcohol oxidase genes on a ω-oxidative metabolism pathway are deleted, the fatty aldehyde dehydrogenase genes are optionally deleted, and β-oxidative metabolism pathway-related genes are deleted, and to a method for producing medium chain diol from fatty acid-derived alcohol or alkane by culturing the recombinant microorganisms. The recombinant microorganisms of the present invention can produce a high yield of medium chain diol by preventing further oxidation and β-oxidative metabolism of fatty alcohols.