Recombinant Microorganisms With Enhanced Nitric Oxide Reductase for NO Removal
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Solution Overview
Problem
Existing nitrogen oxide removal technologies, such as SCR and SNCR, generate secondary greenhouse gases and require high energy and catalyst costs, while biological methods using denitrifying bacteria like Paracoccus and Escherichia lack sufficient nitric oxide reductase activity.
Innovation Solution
A recombinant microorganism with genetic modifications, such as overexpression of nitric oxide reductase genes (norV, norW, norB, norC) from Escherichia and Paracoccus, enhances the activity of nitric oxide reductase to convert NO to N2O and further to N2, reducing NO concentration in samples.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If chemical reduction methods (SCR, SNCR) are used to remove nitrogen oxide, then NO removal efficiency is improved, but energy consumption and catalyst costs increase
Solution Approach 1:
The patent replaces chemical reduction methods (SCR, SNCR) with a biological treatment system using recombinant microorganisms. The mechanical/chemical system is substituted with a biological system where genetically modified bacteria perform nitric oxide removal through enhanced nitric oxide reductase activity, eliminating the need for high energy input and expensive catalysts while maintaining removal efficiency
Solution Approach 2:
The patent changes the fundamental parameter of the treatment system from chemical to biological. By modifying the microbial community structure through genetic engineering (overexpressing norV, norW, norB, norC genes), the system achieves effective NO removal under milder conditions without requiring the high temperatures and catalysts needed for chemical methods
2Productivity
If chemical reduction methods (SCR, SNCR) are used to remove nitrogen oxide, then NO removal efficiency is improved, but secondary waste generation increases
Solution Approach 1:
The patent converts the biological metabolism of microorganisms into a beneficial process for NO removal. The natural respiratory activity of the recombinant microorganisms, which would normally consume oxygen and produce CO2, is harnessed to reduce nitric oxide to nitrogen gas through enhanced nitric oxide reductase activity, transforming a potentially harmful chemical process into an environmentally benign biological one
Solution Approach 2:
The patent replaces chemical reduction methods that generate secondary wastes with a biological system using recombinant microorganisms. The biological treatment pathway eliminates the need for chemical catalysts and reagents, thereby preventing the generation of secondary chemical wastes while maintaining effective NO removal
3Object-affected harmful factors
If denitrifying bacteria are used for biological denitrification, then environmental friendliness is improved, but nitric oxide reductase activity is insufficient
Solution Approach 1:
The patent segments the nitric oxide reduction pathway into distinct genetic components (norV, norW, norB, norC genes) and selectively overexpresses these segments in the denitrifying bacteria. This genetic segmentation allows for targeted enhancement of nitric oxide reductase activity while maintaining the overall biological and environmentally friendly nature of the treatment system
Solution Approach 2:
The patent creates a composite biological system by combining multiple genetically modified components within the denitrifying bacteria. The composite expression of multiple nitric oxide reductase gene products results in synergistic enhancement of enzymatic activity, achieving high NO removal efficiency while preserving the environmental benefits of biological treatment
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The recombinant microorganism efficiently converts nitric oxide to nitrous oxide and nitrogen, effectively lowering NO concentrations in industrial wastewater and waste gases, offering a cost-effective and environmentally friendly solution.
Implementation Method 1
nitric oxide reductase (NOR) in the recombinant microorganism
Implementation Method 2
reduce nitrogen oxide to N2 through a dissimilatory reductive process
Implementation Method 3
reduce nitrogen oxide to N2 through a dissimilatory reductive process
Data Source
AI summary
A recombinant microorganism including a genetic modification that increases activity of nitric oxide reductase in the recombinant microorganism, a composition for reducing a concentration of nitric oxide in a sample, the composition including the recombinant microorganism, and a method of reducing a concentration of nitric oxide in a sample, are disclosed.