Recombinant Strain Allose Production via Psicose Epimerase
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Solution Overview
Problem
Current methods for producing allose from fructose are inefficient due to the production of by-products like altrose and require complex purification processes, generating chemical waste, and lack thermal stability and appropriate pH conditions for industrialization.
Innovation Solution
A recombinant strain expressing psicose epimerase and allose isomerase enzymes, derived from Clostridiun scidens, Ensifer adhaerens, or Treponema primitia, is used to convert fructose into allose with high yield, eliminating by-product formation and maintaining stability across various temperature and pH conditions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If rhamnose isomerase from Pseudomonas stutzeri is used to produce allose from psicose, then allose production is achieved, but large amount of altroses are produced as by-products
Solution Approach 1:
The patent changes the enzyme parameters by selecting psicose epimerase from specific organisms (Clostridiun scidens, Ensifer adhaerens, or Treponema primitia) that operate at optimal temperature and pH conditions for industrialization, eliminating altrose by-product formation while maintaining high allose production
Solution Approach 2:
The patent uses fructose as a starting material that can be converted through psicose as an intermediate, replacing the need for direct psicose input and eliminating the by-product issue associated with rhamnose isomerase while maintaining economical production
2Productivity
If chemical synthesis methods are used to produce allose, then allose can be produced, but additional saccharides are produced and complex purification process is required generating chemical waste
Solution Approach 1:
The patent replaces chemical synthesis methods with enzymatic catalysis using psicose epimerase, substituting chemical reagents and complex purification processes with a biological catalyst that operates under mild conditions and produces no harmful waste
Solution Approach 2:
The enzymatic system performs both epimerization and isomerization functions in sequence, with the enzyme naturally catalyzing the conversion through its active site mechanism, eliminating the need for external chemical reagents and complex purification steps
3Productivity
If conventional enzyme methods are used for allose production, then production is achieved, but the enzyme lacks thermal stability and appropriate pH conditions for industrialization
Solution Approach 1:
The patent identifies and utilizes psicose epimerase from thermally stable organisms (Clostridiun scidens, Ensifer adhaerens, or Treponema primitia) that maintain optimal activity at industrialization-appropriate temperatures and pH conditions, resolving the thermal stability issue
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The recombinant strain enables high-yield production of allose without by-products, ensuring thermal stability and appropriate pH conditions for industrial applications, thus enhancing the efficiency and sustainability of the production process.
Implementation Method 1
a psicose epimerase which produces a psicose from a fructose
Implementation Method 2
an allose isomerase which produces an allose from the psicose
Data Source
AI summary
The present invention relates to a recombinant strain for producing an allose from a fructose, a composition for producing an allose which produces an allose from a fructose-containing raw material comprising the strain, and a method for preparing an allose using the same.