Red-Excitable Fluorescent Dyes for Multiplex DNA Sequencing
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Solution Overview
Problem
Current multiplex dye sets for 4-color automated DNA sequencing require blue or blue-green lasers, which are costly and have limited lifetimes, necessitating the development of fluorescent dyes excitable by longer wavelengths to reduce operational expenses and extend equipment lifespan.
Innovation Solution
Development of novel fluorescent dyes with specific chemical structures that are chemically and photoactively stable and can be excited by light with wavelengths above 600 nm, along with energy transfer dye compounds and labeled nucleosides/nucleotides for efficient multiplex DNA analysis.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If blue or blue-green lasers are used to excite fluorescent dyes in multiplex DNA sequencing, then adequate fluorescence emission from all dyes is achieved, but the cost increases and laser lifetime is limited
Solution Approach 1:
The patent changes the excitation wavelength parameter from blue/blue-green (400-490 nm) to red (600-700 nm) by developing fluorescent dyes with shifted absorption spectra. This allows the use of red lasers which have longer lifetime and lower cost while maintaining adequate fluorescence excitation for multiplex DNA sequencing applications
2Productivity
If multiple spectrally distinguishable fluorescent labels are employed for multiplex detection, then independent detection of multiple analytes is achieved, but the requirement for blue or blue-green lasers increases operational costs
Solution Approach 1:
The patent shifts the spectral parameters of the fluorescent dyes to enable excitation at red wavelengths (600-700 nm) while maintaining spectral distinguishability across multiple dyes. This allows multiplex detection capability to be preserved while changing the excitation wavelength parameter to reduce operational costs and extend laser lifetime
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
These dyes enable cost-effective and stable multiplex DNA sequencing by reducing the need for expensive blue or blue-green lasers, enhancing the stability and efficiency of DNA analysis processes.
Implementation Method 1
these dyes enable cost-effective and stable multiplex DNA sequencing by reducing the need for expensive blue or blue-green lasers, enhancing the stability and efficiency of DNA analysis processes
Implementation Method 2
Fluorescent dye compounds, conjugates and uses thereof
Data Source
AI summary
The present teachings generally relate to fluorescent dyes, linkable forms of fluorescent dyes, energy transfer dyes, reagents labeled with fluorescent dyes and uses thereof.