Reducing Power Analysis Preventing Peak Wavelength Shift
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Solution Overview
Problem
The existing reducing power analysis method using a dye reagent with trivalent iron ions and cyanide suffers from peak wavelength shifts in the optical signal, leading to unreliable quantitative analysis results due to interference from components like albumin and methionine in the sample.
Innovation Solution
A reducing power analysis method involving a reduction step at a pH of 2.4 or lower for the dye reagent containing a ferric compound and cyanide, followed by optical measurement at the peak wavelength of the reduced substance, using a strongly acidic pH adjuster to prevent peak wavelength shifts.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If the reducing power analysis is performed using a dye reagent containing trivalent iron ions and cyanide, then the reducing power can be measured, but peak wavelength shift occurs in the optical signal leading to incorrect quantitative analysis
Solution Approach 1:
The patent changes the pH parameter of the reaction system to 2.4 or lower (highly acidic condition) to prevent peak wavelength shift. This parameter change stabilizes the optical signal by suppressing interactions between the dye reagent and sample components like albumin and methionine, thereby resolving the contradiction between measurement capability and measurement reliability
2Ease of operation
If the optical measurement is performed without controlling pH, then the measurement process is simple, but peak wavelength shift occurs due to sample components like albumin and methionine
Solution Approach 1:
The patent applies preliminary action by pre-adjusting the pH of the dye reagent to 2.4 or lower before mixing with the sample. This preliminary pH adjustment prevents peak wavelength shift from occurring during the measurement process, maintaining measurement precision while keeping the overall process simple
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach ensures accurate analysis of reducing power by stabilizing the optical signal, reducing errors, and enhancing the reliability of the results in clinical tests and biological samples.
Implementation Method 1
a dye reagent colored blood red by a reaction between trivalent iron ions and a cyanide fades when reduced
Implementation Method 2
a dye reagent colored blood red by a reaction between trivalent iron ions and a cyanide fades when reduced
Implementation Method 3
reducing a dye reagent containing a ferric compound and a cyanide at a pH of 2.4 or lower in the presence of a sample
Data Source
Figure 1~2
Figure 3(A)~3(C)
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AI summary
An object of the present invention is to provide a reducing power analysis method that can prevent peak wavelength shift in a sample. In order to achieve the above object, the reducing power analysis method of the present invention includes: a reduction step of reducing a dye reagent containing a ferric compound and a cyanide at pH conditions of 2.4 or lower in the presence of a sample; and an optical measurement step of optically measuring a peak wavelength of a reduced substance obtained in the reduction step.