Reflective Tissue Microscope for Simultaneous Bright- and Dark-Field Imaging
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Solution Overview
Problem
Existing microscopes require multiple steps and risk object movement to obtain both bright-field and dark-field images, especially for biological tissue analysis, and struggle with large-area and label-free analysis.
Innovation Solution
A bright- and dark-field microscope that uses a reflective configuration with a white light source, a ring-forming optical system, and a detachable white scattering plate to obtain both bright-field and dark-field images simultaneously through a single optical path, allowing for easy switching between image types.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If a transmission bright-field microscope is used for biological tissue analysis, then the imaging process requires multiple steps including replacing the condenser lens to obtain dark-field images, but this increases the risk of object movement and reduces operational convenience
Solution Approach 1:
The patent combines both bright-field and dark-field illumination systems within a single microscope body, allowing both imaging modes to be achieved without replacing the condenser lens. The beam splitter and multiple light sources enable simultaneous access to both imaging techniques, eliminating the need for separate transmission microscope operations.
Solution Approach 2:
The microscope is designed with multi-functionality to perform both bright-field and dark-field imaging, as well as reflection imaging, using a unified optical system. The beam splitter and configurable light sources allow the single device to adapt to different imaging requirements without requiring separate specialized microscopes.
2Area of stationary object
If conventional microscopes are used for large-area analysis, then the field of view is limited, but increasing magnification reduces the observable area
Solution Approach 1:
The patent employs a motorized stage with automated positioning capabilities that can dynamically adjust and scan across large areas. This allows the system to maintain high magnification for detailed imaging while automatically moving to capture different regions, effectively combining high resolution with large-area coverage through coordinated stage movement and image stitching.
3Measurement precision
If staining techniques are used for biological tissue analysis, then tissue structure visibility is improved, but the analysis process becomes more complex and time-consuming
Solution Approach 1:
The patent utilizes the inherent optical properties of biological tissues, including natural light absorption and scattering characteristics, to generate contrast without chemical staining. The dark-field illumination enhances the visibility of tissue structures by detecting scattered light from cellular features, eliminating the need for complex staining protocols while maintaining structural visibility.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables convenient, high-quality, and label-free imaging of biological tissues with both bright-field and dark-field images, facilitating large-area analysis and reducing the risk of object movement or damage.
Implementation Method 1
a light source (110); a collimator (120) making light emitted from the light source (110) into parallel light
Implementation Method 2
a through-hole mirror (140) reflecting light coming from the ring-forming optical system (130) to an object (500) and passing light reflecting and coming from the object (500) through a through-hole (145) formed at a center
Implementation Method 3
a reflective objective lens (150) aligning a focus of light reflecting from the through-hole mirror (140) toward the object (500) on the object (500)
Implementation Method 4
a white scattering plate (160) reflecting and scattering light and detachably provided behind the object (500)
Data Source
AI summary
The present disclosure relates to a bright- and dark-field microscope for biological tissue analysis. The object of the present disclosure is to provide a bright- and dark-field microscope, which can obtain both bright-field and dark-field images at the same time and, particularly, can be conveniently used for biological tissue analysis. More specifically, the object of the present disclosure is to provide a bright- and dark-field microscope for biological tissue analysis, the microscope having, as a single optical path and a reflective type, an optical system that can acquire bright-field and dark-field images and, particularly, using white light as a light source so as to even smoothly perform conventional H&E analysis in which a biological tissue is analyzed.