Regenerative Composition Amino Acids Insulin Limb Regeneration

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Solution Overview

Problem

Current methods for inducing limb regeneration in mammals are limited, as they often require species-specific strategies, surgical procedures, or genetic mutations, and lack a universal approach that works across different species and adult mammals.

Innovation Solution

Administration of therapeutically effective amounts of amino acids and insulin, which stimulate mTOR and insulin signaling respectively, to induce reparative and appendage regeneration in subjects, including across multiple species such as moon jellyfish, fruit flies, and adult mice.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If species-specific strategies, surgical procedures, or genetic mutations are used to induce limb regeneration, then regeneration can be achieved in certain species, but the method lacks universality and requires complex species-specific protocols

Engineering Contradiction:

Improveregeneration successVSAvoidspecies universality

Core Design Contradiction:

ReliabilityVSAdaptability or versatility

Solution Approach 1:

The patent applies universality by identifying a common regenerative mechanism (mTOR signaling pathway activation) that functions across multiple species including mammals, birds, and reptiles. The composition containing amino acids and sugars can induce limb regeneration in diverse species without requiring species-specific modifications, surgical procedures, or genetic mutations, thereby achieving both reliability and versatility simultaneously

Inventive Principle:

Principle #6Universality (Multi-functionality)

2Reliability

If complex surgical procedures or genetic mutations are employed to induce regeneration, then regeneration can be achieved, but the procedure complexity and difficulty increase

Engineering Contradiction:

Improveregeneration successVSAvoidprocedure complexity

Core Design Contradiction:

ReliabilityVSDevice complexity

Solution Approach 1:

The patent replaces complex mechanical/surgical procedures and genetic manipulation with a biochemical approach. Instead of performing surgical interventions or genetic mutations, the invention uses a composition of amino acids and sugars that activates the mTOR signaling pathway to induce regeneration, thereby achieving reliable regeneration outcomes with minimal procedural complexity

Inventive Principle:

Principle #28Mechanics substitution (Replace mechanical system)

Solution Approach 2:

The patent changes the biochemical parameters (amino acid and sugar concentrations) to activate the mTOR signaling pathway. By adjusting these chemical parameters rather than employing complex surgical or genetic procedures, the invention achieves effective limb regeneration with simple administration protocols

Inventive Principle:

Principle #35Parameter changes

Data Source

PatentUS20220016127A1Compositions and methods for inducing appendage and limb regeneration

Publication Date: 2022.01.20 CALIFORNIA INST OF TECH

AI summary

Disclosed herein include methods, compositions, and kits suitable for use in inducing reparative regeneration and/or appendage regeneration. In some embodiments, the method comprises administering to a subject in need thereof a therapeutically effective amount of one or more amino acids and a therapeutically effective amount of one or more sugars, and thereby inducing reparative regeneration or appendage regeneration in the subject. There are provided, in some embodiments, regenerative agents suitable for use in the methods provided herein. Regenerative agents can stimulate mTOR signaling and/or insulin signaling. Regenerative agents can include one or more amino acids, insulin, and/or one or more sugars.