Relative Calibration Curve Database for GC/MS Quantitation
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Solution Overview
Problem
The existing method for quantitating substances using chromatographic mass spectrometers requires a significant amount of internal standard substances and frequent use, which is time-consuming, costly, and sometimes difficult to secure, especially when analyzing a large number of samples.
Innovation Solution
A method that uses a relative calibration curve to calculate the concentration of a substance based on the peak area or height ratio in a chromatogram, allowing for the use of a single internal standard substance measurement across multiple samples, reducing the need for frequent additions and minimizing the amount used.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If a standard substance measurement method is used to prepare calibration curves for every GC/MS apparatus, then measurement precision is improved, but device complexity and time consumption increase
Solution Approach 1:
The patent uses a database storing calibration curves obtained from standard substance measurements as a reference model. Instead of performing actual standard substance measurements for each GC/MS apparatus, the system copies and applies pre-established calibration curves from the database, eliminating repetitive calibration procedures while maintaining quantitation accuracy.
Solution Approach 2:
The calibration curves are prepared in advance and stored in a database before actual sample analysis. This preliminary action allows the system to skip the time-consuming standard substance measurement step during routine analysis, as the calibration data is already available for direct application.
2Measurement precision
If internal standard substances are added to each sample for quantitation, then measurement precision is improved, but loss of time and loss of substance increase
Solution Approach 1:
The patent extracts and removes the internal standard substance addition step from the sample preparation process. By using a database with pre-established calibration curves that account for instrument variability, the system eliminates the need to add internal standards to each sample, significantly reducing preparation time and material consumption.
Solution Approach 2:
The database of calibration curves serves as a universal reference that can be applied across multiple samples and different GC/MS apparatus without requiring sample-specific internal standard additions. This universal calibration approach replaces the need for individual internal standard preparation for each sample.
3Measurement precision
If internal standard substances are frequently used for multiple samples, then measurement precision is maintained, but cost and difficulty of securing substances increase
Solution Approach 1:
The patent replaces expensive, difficult-to-obtain internal standard substances with a digital database of calibration curves that has no material consumption. The calibration data acts as a virtual reference that can be reused indefinitely without depleting physical resources, eliminating the ongoing cost and procurement challenges of internal standards.
Solution Approach 2:
The system changes from using physical internal standard substances to using digital calibration curve data. This parameter change from material-based to information-based calibration maintains measurement precision while eliminating the need for continuous internal standard substance procurement and management.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach reduces the amount and frequency of internal standard substance use, lowers costs, and minimizes potential human health risks, enabling efficient quantitation of substances without the need for separate internal standards in each sample.
Implementation Method 1
first, substances to be measured included in a substance (sample) that is the object of analysis are separated along the time axis in a gas chromatographic apparatus (GC)
Implementation Method 2
the respective substances to be measured separated along the time axis are measured using a mass spectrometer (MS) so that the substances to be measured are separated according to the mass number
Data Source
AI summary
A method for quantitating a substance to be measured including: storing a database with which a relative calibration curve is registered; measuring an internal standard substance having a known concentration; calculating the peak area or the height of an internal standard substance by preparing a chromatogram on the basis of a mass spectrum of the internal standard substance; gaining a mass spectrum of the substance to be measured by measuring the substance; calculating the peak area or the height of the substance by preparing a chromatogram on the basis of the mass spectrum of the substance to be measured; and of calculating the concentration of the substance on the basis of the relative calibration curve, the peak area or the height of the internal standard substance and the peak area or the height of the substance.