Removing Polyphenol Contaminants from Enzyme Solutions
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Solution Overview
Problem
Existing methods for producing native polyphenols from feedstock materials are hindered by contaminant phenols introduced during enzymatic processing, which foul and denature the native polyphenols, making it difficult to achieve high purity and value for subsequent applications such as nutraceuticals.
Innovation Solution
A method involving the removal of polyphenols from commercial enzyme solutions before their interaction with feedstock, followed by combining the purified enzyme solution with the feedstock and separating the native polyphenols to produce a pure mixture free of contaminating phenols.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If polyphenols are added to enzyme solutions as stabilizers, then enzyme stability is improved, but contaminant phenols are introduced that foul and denature native polyphenols
Solution Approach 1:
The patent removes contaminant phenols from enzyme solutions through extraction processes before the enzyme solution contacts feedstock. This extraction step separates the harmful polyphenol stabilizers from the enzyme preparation, allowing the enzyme to maintain stability through alternative means while preventing contamination of native polyphenols in the final product.
Solution Approach 2:
The patent applies preliminary treatment to enzyme solutions to remove contaminant phenols before they can interact with and denature native polyphenols. This pre-cleaning step ensures that when the enzyme solution is later added to feedstock, the native polyphenols remain uncontaminated and can be harvested in pure form.
2Reliability
If commercial enzyme solutions with polyphenol stabilizers are used directly, then enzyme performance is maintained, but purification of native polyphenols becomes difficult
Solution Approach 1:
The patent segments the enzyme treatment process into distinct stages: first treating the enzyme solution to remove polyphenol stabilizers, then adding the cleaned enzyme to feedstock, and finally purifying native polyphenols from the treated feedstock. This segmentation allows each step to be optimized independently, maintaining enzyme performance while enabling high-purity native polyphenol recovery.
Solution Approach 2:
The patent introduces an intermediary treatment step that processes the enzyme solution between storage and application. This intermediary step removes contaminant phenols while preserving enzyme activity, serving as a bridge that allows commercial enzyme solutions to be used effectively without compromising the purity of final polyphenol products.
3Duration of action of stationary object
If polyphenol contaminants are present in enzyme solutions, then enzyme storage stability is improved, but the value and effectiveness of harvested polyphenols for nutraceutical applications is reduced
Solution Approach 1:
The patent employs a disposable pre-treatment step for enzyme solutions that removes polyphenol stabilizers before use. This one-time treatment ensures that enzyme storage stability requirements are met during storage, while the harvested polyphenols remain pure enough for high-value nutraceutical applications, effectively separating the storage phase requirements from the product quality requirements.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach allows for the harvesting of native polyphenols in an uncontaminated form, enhancing their value and effectiveness for human health benefits like improved cholesterol levels and cardiovascular health.
Implementation Method 1
The enzyme solution is contacted with activated carbon or a metal impregnated material to remove the polyphenols
Implementation Method 2
The enzyme solution is contacted with activated carbon or a metal impregnated material to remove the polyphenols
Data Source
AI summary
Provided is a method of producing a mixture of pure feedstock-based native polyphenols from a feedstock. Contaminant polyphenols are first removed from an enzyme solution for converting feedstock to a product to produce a polyphenol reduced enzyme solution. The polyphenol reduced enzyme solution is combined with the feedstock and the feedstock is converted to a product and by-product. Heretofore, there has been no process available to reduce or remove the contaminant phenols introduced to the feedstock by commercial enzyme solutions. This method allows for the removal of contaminant phenols prior to introduction to the processing stream and subsequent harvesting of pure feedstock 6 based native polyphenols. The pure feedstock-based polyphenols are removed from the product or by-product to produce a pure mixture of feedstock-based polyphenols.