Replaceable Cuvette Assembly for Optical Absorbance Measurement

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Solution Overview

Problem

Current spectroscopic systems face challenges in measuring hemoglobin parameters in whole blood due to strong optical scattering, which leads to light loss and nonlinear absorbance, requiring improved light collection, increased upper absorbance measurement range, and the use of new algorithms to account for blood settling effects and non-uniform light sources.

Innovation Solution

A replaceable cuvette assembly is designed for installation in a compact COOx analyzer subsystem, featuring a light-emitting module, optical fiber, spectrometer module, and processor module, with diffusers to equalize spatial light distribution, a prism-based spectrometer for low stray light, and a thermal-compensating lens mount to maintain spectral accuracy, and a computational mapping function to process absorbance data.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Measurement precision

If conventional spectroscopic systems are used to measure whole blood, then the measurement process is simple, but strong optical scattering causes light loss and nonlinear absorbance leading to measurement errors

Engineering Contradiction:
Improvehemoglobin parameter measurement accuracyVSAvoidoptical scattering
Core Design Contradiction:
Measurement precisionVSObject-affected harmful factors

Solution Approach 1:

The patent introduces diffusers as intermediary elements positioned between the light source and cuvette, and between the cuvette and spectrometer. These diffusers scatter and redistribute light to create a more uniform spatial light distribution, mediating the interaction between the structured light source and the highly scattering whole blood sample, thereby reducing measurement errors caused by optical scattering

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent changes the spatial distribution parameter of light by using diffusers to transform structured light into more uniform light distribution. This parameter change in light spatial characteristics helps mitigate the effects of optical scattering in whole blood, improving measurement accuracy without requiring complex computational corrections

Inventive Principle:
Principle #35Parameter changes

2Measurement precision

If light collection is improved to overcome scattering, then measurement accuracy improves, but system complexity increases

Engineering Contradiction:
Improveabsorbance measurement accuracyVSAvoidoptical system complexity
Core Design Contradiction:
Measurement precisionVSDevice complexity

Solution Approach 1:

The diffusers serve as simple intermediary optical elements that improve light collection efficiency and uniformity without requiring complex optical systems. The diffusers are positioned strategically in the optical path to naturally redistribute light, achieving improved measurement accuracy through simple additive components rather than complex optical design

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The patent applies local quality improvement by enhancing light distribution specifically in the regions where it matters most for measurement - between the light source and cuvette, and between the cuvette and spectrometer. The diffusers create locally optimized light fields that improve measurement accuracy without requiring global system redesign

Inventive Principle:
Principle #3Local quality

3Measurement precision

If diffusers are added to equalize spatial light distribution, then measurement accuracy improves, but light loss increases

Engineering Contradiction:
Improvespectral response accuracyVSAvoidlight intensity
Core Design Contradiction:
Measurement precisionVSLoss of energy

Solution Approach 1:

The diffusers are designed as intermediary elements that redistribute rather than absorb light. By positioning diffusers at strategic locations in the optical path and using appropriate diffuser materials and geometries, the system achieves uniform light distribution while minimizing light loss through the intermediaries

Inventive Principle:
Principle #24Intermediary (Mediator)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

This solution enables accurate measurement of whole-blood hemoglobin and bilirubin parameters by reducing measurement errors, increasing data collection efficiency, and maintaining spectral response stability, even in the presence of strong optical scattering and blood settling effects.

Implementation Method 1

with diffusers to equalize spatial light distribution

Methodology Applied
Scientific EffectLight scattering/diffusion: Scattering

Implementation Method 2

a prism-based spectrometer for low stray light

Methodology Applied
Scientific EffectLight dispersion: Dispersion (of waves)

Implementation Method 3

optical fiber, spectrometer module

Methodology Applied
Scientific EffectOptical fiber transmission: Optical Fibre

Implementation Method 4

measuring whole-blood hemoglobin parameters or whole-blood bilirubin parameters using optical absorbance measurements

Methodology Applied
Scientific EffectAbsorption spectroscopy: Absorption Spectroscopy

Data Source

PatentEP4071460B1A replacable cuvette assembly
Publication Date: 2024.09.11 NOVA BIOMEDICAL CORP
  • EP4071460B1 patent drawingFigure 1
  • EP4071460B1 patent drawingFigure 2~3B
  • EP4071460B1 patent drawingFigure 4~6

AI summary

A cuvette assembly (40) capable of installation in an optical absorbance measurement system for measuring whole-blood hemoglobin parameters orwhole-blood bilirubin parameters, the cuvette assembly (40) comprising: a cuvette substrate (41); and a cuvette module (43) fixedly connected to the cuvette substrate (41), wherein the cuvette substrate (41) is a support for securing the cuvette assembly (40) within the optical absorbance measurement system, the cuvette module (43) comprising: a sample Inlet port (46); a sample outlet port (47); an electronic chip assembly (48); a sample receiving chamber (54) that fluidly communicates with the sample inlet port (46) and the sample outlet port (47); a first cuvette window (49); and a second cuvette window (52) forming a portion of the sample receiving chamber (54), wherein the first cuvette window (49) and the second cuvette window (52) are aligned with each other, thereby defining a cuvette optical path length between the first cuvette window (49) and the second cuvette window (52), wherein the cuvette module (43) includes a cuvette first portion (44) and a cuvette second portion (50) bonded to each other and thereby form the sample receiving chamber (54), and wherein the first cuvette window (49) and the second cuvette window (52) are disposed within an optical path of the optical absorbance measurement system.