Resin Layer Testing Device for Antibody Fixation and Color Development
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Solution Overview
Problem
Conventional testing devices face issues with the interaction between reagents and the flow path member, leading to ineffective diffusion and fixation of reagents, resulting in poor utilization of capture antibodies and inefficient color development due to light scattering in hydrophilic porous materials.
Innovation Solution
A testing device with a porous flow path member and resin layers, where reagents are provided as a solid phase over the surface of the resin layers, allowing for adjustable interaction and fixation, enhancing the release and fixation of reagents and improving color development efficiency.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Speed
If a hydrophilic porous material is used as the flow path member to increase sample spreading speed, then the sample diffusion speed is improved, but light scattering occurs preventing effective sensing of colorant from labeling particles
Solution Approach 1:
The flow path member is divided into two distinct functional zones: a hydrophilic porous material region for rapid sample transport, and a resin layer region for reagent fixation and color development. This segmentation allows each zone to optimize its specific function without interfering with the other.
Solution Approach 2:
The resin layer acts as an intermediary between the hydrophilic porous material and the detection system. It receives the sample from the porous material, provides a light-transmissive environment for colorant sensing, and enables effective antibody-antigen reactions without the light scattering problems of the porous material.
2Quantity of substance
If reagents are directly applied over the hydrophilic porous material, then the capture antibody is present in the material, but the antibody diffuses and cannot be effectively utilized
Solution Approach 1:
The capture antibody is pre-fixed to the resin layer before sample application. This preliminary fixation ensures the antibody is positioned optimally in a light-transmissive environment and prevents diffusion during sample flow, maximizing utilization efficiency.
Solution Approach 2:
The resin layer changes the physical and optical parameters of the reagent environment compared to hydrophilic porous material. It provides appropriate hydrophobicity for antibody fixation and light transmissivity for colorant detection, optimizing both antibody utilization and signal detection.
3Speed
If arbitrary flow path member material is selected to increase sample spreading speed, then diffusion speed is improved, but interaction with reagents becomes excessively strong or weak
Solution Approach 1:
The flow path member is segmented into a hydrophilic porous material portion for speed optimization and a resin layer portion for controlled reagent interaction. This allows independent optimization of each function without compromise.
Solution Approach 2:
Different regions of the flow path member have different properties: the hydrophilic porous material region provides high water absorption and rapid sample transport, while the resin layer region provides controlled hydrophobicity for optimal reagent interaction. Each region is optimized for its specific local function.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The device enables controlled release and fixation of reagents, improving the efficiency of color development and reducing the amount of capture antibodies needed, with clear and intense line visibility and density, compared to conventional methods.
Implementation Method 1
a reagent reactive with the sample is provided as a solid phase over a surface of the resin layer facing the flow path member
Implementation Method 2
a porous flow path member in which a flow path for flowing a sample is formed
Data Source
AI summary
Provided is a testing device, including: a porous flow path member in which a flow path for flowing a sample is formed; and a resin layer provided at one position or a plurality of positions over the flow path member, wherein a reagent reactive with the sample is provided as a solid phase over a surface of the resin layer facing the flow path member.