Reversible Polymer-Antibody Labels for Cell Viability
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Solution Overview
Problem
Current methods for removing magnetic labels from cells in immunomagnetic cell separation are either destructive to cells, damage cell viability, or are overly complex and time-consuming.
Innovation Solution
The use of a low-avidity, high-affinity biomolecular interaction system involving polymers and anti-polymer ligands, where biological targets are linked with polymers and released using physiologically compatible polymeric compounds.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional methods are used to remove magnetic labels from cells, then the labels can be removed, but the cell viability is damaged or the process becomes overly complex and time-consuming
Solution Approach 1:
The patent employs a reversible binding system where the interaction parameters between the label and cell can be dynamically changed. By using a competitive elution mechanism, the binding affinity is temporarily reduced to enable label removal, then restored to maintain cell viability for subsequent applications
Solution Approach 2:
The patent introduces an intermediary elution buffer that mediates the label removal process. This buffer contains components that temporarily interfere with the label-cell interaction, allowing label detachment without direct exposure of cells to harsh conditions that would damage viability
2Reliability
If conventional methods are used to remove magnetic labels from cells, then the labels can be removed, but the process becomes overly complex
Solution Approach 1:
The patent extracts the label removal function into a simple, standardized elution buffer formulation that can be applied universally across different label-cell systems. This eliminates the need for complex, system-specific removal protocols and simplifies the overall process while maintaining cell viability
Solution Approach 2:
The elution buffer is designed with universal applicability, containing components that can disrupt various label-cell interactions through a common mechanism. This multi-functional approach allows a single buffer formulation to handle diverse labeling scenarios without requiring complex, customized removal procedures
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method allows for rapid and efficient removal of magnetic labels from cells under mild conditions, maintaining cell viability and simplifying the process, while being broadly applicable across different cell types and species.
Implementation Method 1
Magnetic microparticles or nanoparticles are targeted to cell receptors using the affinity binding characteristics of proteins or antibodies
Implementation Method 2
magnetic labeling enables the imaging of biological targets using magnetic resonance imaging (MRI) or medical particle imaging (MPI) techniques
Implementation Method 3
fluorescent labeling enables the visualization of biological targets with, in some cases, molecular sensitively
Implementation Method 4
magnetic microparticles or nanoparticles are targeted to cell receptors using the affinity binding characteristics of proteins or antibodies
Data Source
AI summary
This disclosure provides compositions and methods for a low-avidity, high-affinity and high-specificity biomolecular interaction that is rapidly reversible under physiological conditions. The methods comprise linking biological targets (such as molecules, proteins, DNA, cells, etc.) with polymers and anti-polymer ligands and a way to reverse their binding using physiologically compatible polymeric compounds. The methods also comprise a way to combine different polymer/anti-polymer systems for orthogonal labeling. The compositions comprise labels including particles (fluorescent, magnetic, dense, etc.) conjugated to polymers or labels conjugated to anti-polymer antibodies. The compositions also comprise biomolecules (proteins, antibodies, DNA, etc.) conjugated to the polymers. These methods and compositions represent a major improvement to the state-of-the-art. They are particularly useful for separation and isolation of biological targets using particles, but have important application to other fields including fluorescent imaging.