Reversible Seal Flow Cell for In Situ Sequencing
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Solution Overview
Problem
Existing sample analysis devices face challenges in providing precise reagents and controlling sample quality for in situ sequencing and hybridization, requiring improved methods and devices for sample manipulation and imaging.
Innovation Solution
A sample device comprising a first and second layer that reversibly seal to form a flow path with a window for imaging, allowing for precise control of reagents and sample manipulation, featuring a thickness range of 1 μm to 1000 μm and a hydrophobic pattern for fluid management, enabling enhanced mechanical stability and ease of assembly.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If a gasket is used to seal the flow path, then the seal reliability is improved, but the device complexity and assembly difficulty increase
Solution Approach 1:
The patent removes the gasket component from the flow cell assembly, extracting the sealing function from a separate component and integrating it directly into the layer structure through reversible seals, thereby reducing device complexity while maintaining seal reliability
Solution Approach 2:
The sealing function is merged with the layer structure itself through reversible seals formed between layers, combining the sealing function with the structural components rather than using a separate gasket component, simplifying the overall device design
2Strength
If the window thickness is increased for mechanical stability, then the structural strength is improved, but the optical imaging quality deteriorates
Solution Approach 1:
The patent applies different thickness characteristics to different regions: the window region maintains thinness (1-1000 μm) for optimal optical imaging, while other structural regions provide necessary mechanical support, allowing each region to have the quality needed for its specific function
Solution Approach 2:
The flow cell uses composite layer structures combining materials with different properties, where the window layer prioritizes optical transparency and thinness, while supporting layers provide mechanical strength, creating a composite structure that satisfies both optical and mechanical requirements
3Reliability
If the flow path is sealed permanently, then the seal reliability is improved, but the ease of repair and reusability worsen
Solution Approach 1:
The patent employs reversible seals that can dynamically transition between sealed and unsealed states, allowing the flow cell to be assembled and disassembled multiple times while maintaining reliable sealing during use, thus enabling both reliability and ease of repair through dynamic seal behavior
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The device provides a durable, easy-to-assemble, and optically optimized platform for sample analysis, ensuring precise reagent delivery and imaging while maintaining a fluid-tight seal without the need for a gasket, enhancing mechanical stability and handling robustness.
Implementation Method 1
The first and second layers are configured to seal via an adhesive, conformal contact, or capillary force.
Implementation Method 2
The first and second layers are configured to seal via an adhesive, conformal contact, or capillary force.
Implementation Method 3
The flow path is bounded in part by a hydrophobic pattern on the first and/or second layer.
Data Source
AI summary
The present invention features devices and methods for supplying a biological sample with a fluid. In various embodiments, a device includes a first layer and a second layer. The first layer includes a window having a thickness of about 1 μm to about 1000 μm. The first and second layers reversibly seal to form a flow path having an inlet and an outlet and bounded in part by the window.