RFID-Integrated Organoids for Traceable Tissue Culture
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Existing methods fail to incorporate RFID microchips into biological tissues without impairing their native structure and functions, limiting their application in tissue culture contexts.
Innovation Solution
Self-assembly mediated incorporation of ultracompact RFID sensors into organoids, allowing for wireless traceability and maintaining native tissue functions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If RFID microchips are incorporated into biological tissues, then tracking capability is improved, but tissue structure and function are impaired
Solution Approach 1:
The RFID tracking system is segmented into ultra-small microchips (1mm or smaller) that can be individually incorporated into organoids without compromising the overall tissue structure. The segmentation allows distributed tracking points within the tissue while maintaining native tissue architecture and function.
Solution Approach 2:
The RFID microchips are localized to specific positions within the organoid tissue, with each chip occupying a minimal local volume. This local placement strategy enables tracking capability at specific points while leaving the rest of the tissue structure and functionally intact.
2Loss of information
If conventional RFID chips are used in tissue culture, then identification capability is improved, but tissue viability is worsened
Solution Approach 1:
The patent uses ultra-small RFID microchip copies or replicas that replicate the essential identification functionality of conventional RFID chips but with dramatically reduced size and minimal biological impact. These microchip copies provide the same identification capability while being biocompatible with tissue culture environments.
Solution Approach 2:
The RFID chip size parameter is changed from conventional dimensions to ultra-small scales (1mm or smaller), and the material composition parameters are optimized for biocompatibility. These parameter changes enable the chips to function for identification while minimizing harmful effects on tissue viability.
3Loss of information
If RFID sensors are integrated into organoids, then traceability is improved, but organoid complexity increases
Solution Approach 1:
The RFID sensing functionality is extracted as a separate, self-contained microchip component that is incorporated into the organoid. This extraction allows the traceability function to be added without fundamentally altering the organoid's biological structure, as the microchip operates as an independent element within the tissue.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The RFID-integrated organoids (RiO) maintain their structure and function, enabling in vitro and in vivo tracking, phenotypic screening, and cryopreservation, with successful donor identification and phenotypic analysis.
Implementation Method 1
Self-assembly mediated incorporation of ultracompact RFID sensors into organoids
Data Source
AI summary
Disclosed are digitized organoids comprising a detectable sensor, such as, for example, a Radio frequency identification (RFID) based sensor. Further disclosed are methods for making the digitized organoids. The disclosed methods allow for self-assembly mediated incorporation of ultracompact RFID sensors into organoids. Methods of using the digitized organoids are also disclosed.