Rhodosorus Marinus Extract Purification for Antioxidant Facial Masks
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Solution Overview
Problem
Conventional facial masks provide insufficient deep nourishment, especially for those requiring additional moisturizing and anti-aging effects, and there is a need for more effective skincare ingredients that combat free radicals and improve skin elasticity.
Innovation Solution
Utilizing Rhodosorus marinus ZS2001, specifically its phycoerythrin and active peptide, through a purification process involving centrifugation, ultrasound, ammonium sulfate treatment, and enzymolysis, to create a facial mask formulation with Rhodosorus marinus extract, squalane, collagen, and other ingredients.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional facial masks based on water, gel and emulsion are used, then the basic skincare function is provided, but the deep nourishment and anti-aging effects are insufficient
Solution Approach 1:
The patent creates a composite facial mask formulation by combining Rhodosorus marinus extract (containing phycobiliproteins), collagen, hyaluronic acid, and various skincare ingredients in specific proportions. This composite approach enables synergistic effects that provide deep nourishment and anti-aging benefits superior to conventional single-base formulations.
Solution Approach 2:
The patent optimizes formulation parameters including the concentration of Rhodosorus marinus extract (1-10%), collagen (0.1-5%), and hyaluronic acid (0.1-5%) to achieve optimal balance between deep nourishment effectiveness and manufacturing feasibility, resolving the contradiction between performance and ease of manufacture.
2Reliability
If Rhodosorus marinus extract with high antioxidant activity is used, then free radical scavenging and anti-aging effects are enhanced, but the purification process complexity increases
Solution Approach 1:
The patent extracts and isolates specific active components (phycobiliproteins, collagen, hyaluronic acid) from Rhodosorus marinus through a systematic purification process involving centrifugation, filtration, and chromatography. This extraction approach concentrates the antioxidant activity while managing process complexity through staged purification.
Solution Approach 2:
The purification process is segmented into multiple discrete steps: cell lysis, centrifugation at different speeds, filtration through specific membranes, and chromatographic separation. This segmentation allows each step to be optimized independently, managing overall process complexity while achieving high antioxidant activity.
3Manufacturing precision
If multiple purification steps (centrifugation, ultrasound, ammonium sulfate treatment, enzymolysis) are performed, then the purity and efficacy of active peptides are improved, but the processing time and energy consumption increase
Solution Approach 1:
The patent performs preliminary actions including pre-freezing the Rhodosorus marinus culture, performing initial centrifugation at lower speeds before final purification, and conducting ultrasound treatment before ammonium sulfate precipitation. These preliminary steps prepare the sample efficiently, reducing the time and energy required for subsequent purification steps while maintaining high active peptide purity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The facial mask demonstrates significant antioxidant activity, enhancing skin moisturization, reducing wrinkles, and improving skin elasticity, offering superior nourishment and anti-aging benefits.
Implementation Method 1
taking fresh Rhodosorus marinus to undergo a first centrifugation, soaking the centrifuged Rhodosorus marinus in water, performing ultrasound on the soaked Rhodosorus marinus, followed by a second centrifugation to collect supernatant
Implementation Method 2
performing ultrasound on the soaked Rhodosorus marinus
Implementation Method 3
purifying the crude phycoerythrin extract obtained in step (1) once using a saturated ammonium sulfate method to obtain a preliminarily purified phycoerythrin
Implementation Method 4
adding an appropriate amount of neutral protease, followed by enzymolysis at 40-50° C. for 4-6 h to obtain an enzymatic hydrolysate
Data Source
AI summary
The present disclosure relates to Rhodosorus marinus and an application thereof in preparing facial masks. Rhodosorus marinus ZS2001 has the following collection information: collection institution: China Center for Type Culture Collection (CCTCC); address of the collection institution: Wuhan University, No. 299 Bayi Road, Wuchang District, Wuhan City, Hubei Province; collection date: Oct. 30, 2023; collection number: CCTCC NO: M20232086; and collection name: Rhodosorus marinus.