Rice EPSPS Mutant Glyphosate Resistance Non-Transgenic
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Solution Overview
Problem
Current glyphosate-resistant crops rely heavily on transgenic methods using genes from microorganisms, which face low acceptance and limited application in various crops due to public perception and genetic modification concerns.
Innovation Solution
Development of a rice EPSPS mutant with specific amino acid mutations that confers glyphosate resistance, allowing for the creation of glyphosate-resistant plants using a native rice gene, enabling broader crop resistance without transgenic technology.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If transgenic methods using genes from microorganisms are used to create glyphosate-resistant crops, then glyphosate resistance is achieved, but public acceptance and application scope are limited
Solution Approach 1:
The patent applies parameter changes by introducing specific amino acid mutations at positions 70, 111, 116, 152, 213, 250, 252, and 342 of the rice EPSPS protein sequence. These parameter changes in the protein structure confer glyphosate resistance while maintaining the enzyme's catalytic activity, thereby achieving both reliability of resistance and adaptability across different crop types without relying on transgenic approaches from microorganisms
2Reliability
If transgenic methods using genes from microorganisms are used to create glyphosate-resistant crops, then glyphosate resistance is achieved, but public acceptance is low
Solution Approach 1:
The patent inverts the conventional approach by instead of introducing foreign microbial genes into crops, it modifies the native rice EPSPS gene through site-directed mutagenesis. This inversion from transgenic to non-transgenic approach maintains glyphosate resistance reliability while significantly improving ease of manufacture in terms of public acceptance and regulatory approval
3Ease of manufacture
If native rice EPSPS gene is used with amino acid mutations, then public acceptance improves, but glyphosate resistance may be reduced
Solution Approach 1:
The patent carefully selects and optimizes parameter changes at specific amino acid positions (70, 111, 116, 152, 213, 250, 252, 342) to achieve the right balance. These targeted parameter changes confer sufficient glyphosate resistance while maintaining the enzyme's natural properties, thereby ensuring both reliability of resistance and ease of manufacture through non-transgenic approach
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The rice EPSPS mutant maintains bio-enzyme activity and provides high glyphosate resistance in various crops such as rice, tobacco, soybean, maize, wheat, cotton, and sorghum, offering a non-transgenic solution with improved public acceptance.
Implementation Method 1
EPSPS catalyzes PEP and shikimate-3-phosphate to synthesize EPSP in a shikimic acid pathway
Data Source
AI summary
A paddy rice EPSPS mutant, and an encoding gene and use thereof, relating to the technical field of genetic engineering. The paddy rice EPSPS mutant has an amino acid sequence shown as SEQ ID NO. 1. The paddy rice EPSPS mutant has glyphosate resistance and can resist glyphosate having a concentration of 100 mM. The paddy rice EPSPS mutant has very wide use prospect in the field of cultivating glyphosate-resistant plants.