RKD Transcription Factor Crop Yield Enhancement

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Solution Overview

Problem

Current methods for increasing crop yields are slow and lack control, as they rely on selective breeding and expansion of arable land, which can lead to undesirable trait introduction and environmental degradation.

Innovation Solution

Introducing RKD transcription factors or modifying endogenous nucleic acid sequences to increase the expression of these factors in plants, thereby enhancing axillary meristem formation and yield-related traits such as tiller number and seed yield.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Productivity

If selective breeding strategies are used to increase crop yields, then yield-related traits are improved, but the process is slow and lacks control over undesirable traits

Engineering Contradiction:
Improvecrop yieldVSAvoidbreeding time
Core Design Contradiction:
ProductivityVSLoss of time

Solution Approach 1:

The invention changes the fundamental parameter of yield improvement from phenotypic selection through breeding to direct genetic modification. By introducing specific transcription factor genes (RKD family) that control meristem formation, the process transitions from slow multi-generational breeding to direct genetic parameter manipulation, achieving rapid and controlled yield enhancement without time loss.

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The invention uses transcription factors as molecular intermediaries to control yield traits. Instead of direct selective breeding of complex phenotypes, specific RKD transcription factor genes serve as intermediaries that mediate the control of axillary meristem formation, enabling precise and rapid manipulation of yield-related traits through genetic transformation.

Inventive Principle:
Principle #24Intermediary (Mediator)

2Productivity

If arable land is expanded through deforestation to increase food production, then crop production capacity is improved, but environmental degradation and loss of biodiversity occur

Engineering Contradiction:
Improvefood production capacityVSAvoidenvironmental degradation
Core Design Contradiction:
ProductivityVSObject-affected harmful factors

Solution Approach 1:

The invention changes the parameter of production capacity from land area expansion to genetic trait enhancement. By modifying endogenous nucleic acid sequences or introducing RKD transcription factor genes, existing crops achieve higher yields per unit area, eliminating the need for deforestation and land expansion while maintaining environmental integrity.

Inventive Principle:
Principle #35Parameter changes

3Manufacturing precision

If selective breeding is used to introduce preferable traits, then desired yield traits are improved, but undesirable traits are also introduced through random mutation

Engineering Contradiction:
Improvetrait introduction precisionVSAvoidundesirable traits
Core Design Contradiction:
Manufacturing precisionVSObject-generated harmful factors

Solution Approach 1:

The invention extracts the control mechanism for yield traits from complex random mutation processes. By identifying and introducing specific RKD transcription factor genes that directly control axillary meristem formation, the invention isolates the precise genetic determinant of yield, eliminating the introduction of unrelated undesirable traits that occur in conventional breeding.

Inventive Principle:
Principle #2Taking out (Extraction)

Solution Approach 2:

The invention changes the precision parameter from indirect phenotypic selection to direct genetic modification. By targeting specific transcription factor genes with known functions in meristem control, the process achieves precise introduction of desired traits without the random mutation events that generate undesirable traits in traditional breeding.

Inventive Principle:
Principle #35Parameter changes

Data Source

PatentUS20250043300A1Methods to increase yields in crops
Publication Date: 2025.02.06 WARWICK THE UNIV OF
  • US20250043300A1 patent drawing
  • US20250043300A1 patent drawing
  • US20250043300A1 patent drawing

AI summary

The present invention relates to a method of producing a modified plant, part, cell or protoplast thereof having an increase in a yield-related trait relative to a reference plant, part, cell or protoplast thereof. More particularly, the invention relates to providing the plant, part, cell or protoplast thereof with an RKD transcription factor, a polynucleotide encoding the same, or a gene editing system for modifying an endogenous nucleic acid sequence encoding an RKD transcription factor or regulatory sequence thereof. Said plants have improvements in a yield-related trait, and thereby improved yield, which is especially useful in agriculture.