RNA-Anchoring Fluorescent Probe for Long-Window Tumor Imaging
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Solution Overview
Problem
Existing materials for tumor imaging and treatment fail to accumulate in tumor tissues for a long time, reducing their bioavailability and efficacy.
Innovation Solution
A novel red-light-mediated nucleic acid anchoring fluorescent probe is synthesized through specific chemical reactions, enabling prolonged in vivo fluorescence and photoacoustic imaging by cross-linking with RNA in tumor cells.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Duration of action of stationary object
If existing materials are used for tumor imaging and treatment, then the materials can be introduced into tumor tissues, but they cannot accumulate in tumor tissues for a long time, reducing bioavailability
Solution Approach 1:
The probe is designed with a nucleic acid anchoring group that performs preliminary binding to RNA in tumor cells before the therapeutic or imaging function is activated. This preliminary anchoring action ensures long-term retention and accumulation in tumor tissues, solving the problem of short residence time of existing materials.
Solution Approach 2:
The probe uses RNA as an intermediary molecule to mediate the accumulation and retention of the fluorescent probe in tumor tissues. By anchoring to RNA, the probe achieves long-term accumulation without being rapidly cleared, thereby improving both accumulation time and bioavailability.
2Duration of action of stationary object
If the probe accumulates in tumor tissues for a long time, then bioavailability is improved, but the complexity of the probe structure increases
Solution Approach 1:
The probe merges multiple functions into a single molecular structure: fluorescence emission for imaging, photoacoustic conversion for thermal/photothermal therapy, and nucleic acid anchoring for long-term retention. This consolidation achieves long retention time while managing structural complexity through functional integration rather than separate components.
Solution Approach 2:
The probe is designed with multi-functionality, serving as an imaging agent, a therapy agent, and an accumulation agent simultaneously. The universal design allows a single probe molecule to achieve long-term retention in tumor tissues without requiring multiple separate materials, thus balancing retention time with structural complexity.
3Reliability
If the probe uses cross-linking ability to anchor in tumor cells, then imaging and therapy efficacy is improved, but the chemical reaction complexity increases
Solution Approach 1:
The probe employs self-service cross-linking chemistry where the probe molecule automatically anchors to RNA in tumor cells through its inherent nucleic acid anchoring group. This self-anchoring mechanism improves imaging and therapy efficacy without requiring external cross-linking agents or complex chemical reactions, thus maintaining chemical simplicity.
Solution Approach 2:
The probe utilizes parameter changes in the chemical environment of tumor cells (such as pH, ionic strength, or RNA concentration) to trigger the cross-linking or anchoring reaction. This allows the probe to activate its cross-linking ability selectively in tumor cells under physiological conditions, improving efficacy while avoiding complex controlled chemical reactions.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The probe achieves long-term retention in tumor tissues, promotes apoptosis of tumor cells, and inhibits tumor growth through cross-linking with RNA, enhancing imaging time and therapeutic efficacy.
Implementation Method 1
utilizes the advantages of its groups of cross-linking ability... to carry out longstanding in vivo fluorescence
Implementation Method 2
near-infrared emission to carry out longstanding in vivo fluorescence
Implementation Method 3
photoacoustic imaging
Data Source
AI summary
The present invention disclosed a red-light-mediated nucleic acid anchoring fluorescent probe and its preparation method and application. The fluorescent probe has the capability of taking a crosslinking reaction with RNA in cytoplasm under singlet oxygen mediation, and imaging of tumor tissues in a long window period is achieved. Moreover, it is found that by means of the probe, after crosslinking of RNA, a severe apoptosis phenomenon of tumor cells occurs, and the integration of diagnosis and treatment of tumors is achieved.