RNA Membrane Production via Evaporation Concentration
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Solution Overview
Problem
Current methods for manufacturing RNA membranes are costly and result in products that are not exclusively composed of RNA, making them unsuitable for medical applications and difficult to control, especially for bio-organ applications like the pericardium, and they are prone to degradation.
Innovation Solution
A method involving the production of first and second circular DNA, followed by transcription to create long linear RNA strands that are then concentrated through evaporation to form an RNA membrane, allowing for controlled bonding of base pairs and adjustable roughness and thickness, enabling the production of an RNA membrane composed exclusively of RNA.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If RNA is synthesized using conventional methods, then RNA membranes can be produced, but the production cost is excessively high and the RNA is easily degraded
Solution Approach 1:
The patent applies preliminary action by pre-synthesizing circular DNA templates with specific sequences before transcription. These circular DNA templates are designed in advance to produce RNA with enhanced stability, avoiding the need for costly and unstable conventional RNA synthesis methods. The preliminary design of circular DNA with optimized sequences allows for cost-effective production of stable RNA membranes.
Solution Approach 2:
The patent changes the fundamental parameter of RNA synthesis by using transcription from circular DNA templates instead of direct chemical synthesis. This parameter change transforms the production approach from expensive and unstable chemical methods to a more stable and cost-effective biological transcription process, while maintaining RNA purity and functionality.
2Ease of operation
If RNA membranes are produced using existing techniques, then membranes can be formed, but they include cation materials and are not composed exclusively of RNA
Solution Approach 1:
The patent applies the extraction principle by removing cation materials from the RNA membrane composition entirely. Instead of using cationic lipids or other extraneous materials to form membranes, the invention extracts only pure RNA components through transcription from circular DNA templates. This ensures the membrane is composed exclusively of RNA without requiring complex purification steps to remove contaminants.
Solution Approach 2:
The patent uses copying by transcribing RNA sequences from circular DNA templates. This copying process produces identical RNA sequences that self-assemble into membranes, eliminating the need for complex chemical synthesis and ensuring pure RNA composition. The copying mechanism from DNA to RNA provides a straightforward path to purity without adding process complexity.
3Manufacturing precision
If long linear RNA strands are used to form membranes, then base pair bonding can be activated, but the RNA strands must be concentrated effectively
Solution Approach 1:
The patent applies dimensionality change by utilizing the evaporation process to concentrate RNA strands from a three-dimensional solution into a two-dimensional film on the container surface. This dimensional transition automatically concentrates the long linear RNA strands and activates base pair bonding without requiring complex mechanical concentration equipment. The evaporation process naturally controls membrane thickness and uniformity, achieving manufacturing precision through a simple process.
Solution Approach 2:
The patent uses phase transitions by employing evaporation to concentrate RNA strands. The phase change from liquid to vapor removes solvent and concentrates the RNA molecules, enabling them to self-assemble into membranes through base pair bonding. This phase transition approach provides precise control over membrane formation while maintaining manufacturing simplicity, as evaporation is a naturally controllable process.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method allows for the cost-effective production of a non-toxic, controllable RNA membrane that can be reused and stored for long periods, suitable for bio-organ applications and drug delivery, with controlled properties and enhanced stability.
Implementation Method 1
a first linear RNA production step of producing first linear RNA using the first circular DNA through transcription; a second linear RNA production step of producing second linear RNA using the second circular DNA through transcription
Implementation Method 2
an RNA membrane production step of producing an RNA membrane by inducing bonding of base pairs of the first linear RNA and the second linear RNA
Data Source
AI summary
Disclosed are a method of manufacturing an RNA membrane and an RNA membrane manufactured thereby, wherein the RNA membrane can be produced at lower cost, in which the RNA membrane is composed exclusively of RNA, and thus has no toxicity in vivo, is controllable, and can be effectively applied to bio-organs such as the pericardium, as well as the production of peptides or proteins, and particularly, long linear RNA strands, which have not yet formed particles, are concentrated on the surface of a tube by inducing an evaporation process to thus activate the bonding of base pairs thereof, and the roughness and thickness of the RNA membrane can be controlled by changing the manufacturing conditions.