Automatic ROS Detection System for Cell Microenvironments
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Solution Overview
Problem
Current methods for detecting reactive oxygen species (ROS) in microenvironments of cells, tissues, and organs are not capable of rapid, accurate, and automatic detection, especially in large volumes of samples, due to instability of ROS and the need for multiple complex steps, which limits their application in clinical examinations.
Innovation Solution
An automatic ROS detection system incorporating a sample transmission reaction system, washing system, and purge system connected through light-avoiding, water, and gas pipelines, respectively, with a fluorescence detection system using DCFH-DA probes and H2O2 standardization for precise and rapid analysis.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If manual detection methods are used for ROS content, then flexibility in operation is maintained, but detection speed and productivity are low
Solution Approach 1:
The system divides the detection process into distinct functional modules: sample injection unit, reaction unit, detection unit, washing unit, and purge unit. Each module performs a specific function and can be independently controlled, enabling automated high-throughput detection while maintaining manageable system complexity through modular design.
Solution Approach 2:
The system replaces manual mechanical operations with automated fluid control mechanisms. The fluid control unit automatically manages liquid flow between different units through controlled dispensing and aspiration, eliminating manual pipetting and significantly increasing detection speed and productivity.
2Measurement precision
If multiple detection steps are performed manually, then detection accuracy can be maintained, but time consumption increases
Solution Approach 1:
The system performs multiple detection steps continuously without interruption. The fluid control unit maintains continuous liquid flow between units, and the automated sequence ensures that sample injection, reaction, detection, washing, and purging occur in uninterrupted succession, significantly reducing total detection time while maintaining precision through controlled conditions.
Solution Approach 2:
The system performs preliminary preparation of reagents and samples before the actual detection process. The fluid control unit pre-dispenses required volumes of detection reagents into the reaction unit, and the system is pre-configured with all necessary components, allowing the detection process to proceed immediately without delays for manual preparation.
3Reliability
If ROS samples are exposed to air during detection, then oxygenation is maintained, but ROS instability increases and detection accuracy decreases
Solution Approach 1:
The system creates a controlled closed environment where samples are handled within the automated system without exposure to ambient air. The fluid control unit manages all liquid transfers through sealed pathways, and the reaction and detection occur in enclosed units, protecting ROS samples from unwanted oxidation while maintaining detection reliability.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables high sensitivity, accuracy, and speed in detecting ROS levels in microenvironments, suitable for both scientific research and clinical applications, overcoming the limitations of existing methods by ensuring reliable and efficient detection of ROS in large volumes of samples.
Implementation Method 1
The ROS in the cells can oxidize the DCFH without fluorescence to produce DCF with fluorescence, and the reaction between the DCFH and ROS molecules does not have specificity, so the overall level of the ROS in cells can be determined by detecting the fluorescence of the DCF.
Implementation Method 2
The signal obtained by the fluorescence detector is transmitted to a data processing terminal after being converted through a photoelectric converter.
Implementation Method 3
The ROS in the cells can oxidize the DCFH without fluorescence to produce DCF with fluorescence
Data Source
AI summary
The present disclosure relates to a biochemical detection instrument. The technical solution is an automatic reactive oxygen species content detection system suitable for a cell microenvironment that includes: a sample transmission reaction system and a detection system which are communicated in sequence through a light avoiding pipeline. A washing system is in communication with the sample transmission reaction system through a water pipeline, and a purge system is in communication with the sample transmission reaction system through a gas pipeline. The sample transmission reaction system further includes a sample injector and a DCFH supply bin which are communicated with a reaction bin through light avoiding pipelines after being connected in parallel. Sample injection valves are respectively configured between the sample injection valve and the reaction bin and between the DCFH supply bin and the reaction bin.