Simultaneous Extraction of Polysaccharides, Polyphenols, SOD and Vc from Rosa Roxburghii Pomace
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Solution Overview
Problem
Rosa roxburghii Tratt pomace, rich in polysaccharides, polyphenols, SOD, and Vc, is largely underutilized and discarded, leading to resource waste and environmental pollution, with existing methods limited to dietary fiber extraction and other low-value applications.
Innovation Solution
A method and system involving enzymatic hydrolysis, grinding, ultrasonic treatment, ceramic membrane ultrafiltration, macroporous adsorption resin treatment, spiral-wound membrane ultrafiltration, and reverse osmosis to simultaneously extract polysaccharides, polyphenols, SOD, and Vc from Rosa roxburghii Tratt pomace, optimizing conditions for high yield and purity.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Ease of manufacture
If traditional extraction methods are used for dietary fiber and polysaccharide extraction, then the extraction process is simple, but the utilization value is low and most pomace is discarded
Solution Approach 1:
The extraction process is divided into multiple sequential stages: enzymatic hydrolysis treatment, grinding, ultrasonic treatment, centrifugation, ceramic membrane ultrafiltration, macroporous adsorption resin treatment, spiral-wound membrane ultrafiltration, and reverse osmosis. Each stage targets specific components (polysaccharides, polyphenols, SOD, Vc) for selective extraction and purification, transforming a single simple extraction into a multi-stage systematic process that maximizes utilization value.
Solution Approach 2:
The integrated extraction system performs multiple functions simultaneously: it extracts polysaccharides, polyphenols, SOD, and Vc from the same pomace material through different mechanisms (enzymatic hydrolysis, ultrasonic vibration, membrane filtration, adsorption, reverse osmosis). This multi-functional approach converts previously wasted pomace into high-value products while maintaining process efficiency.
2Productivity
If multiple extraction methods are combined to extract polysaccharides, polyphenols, SOD and Vc simultaneously, then the extraction yield and purity are improved, but the process complexity increases
Solution Approach 1:
The complex extraction process is segmented into distinct functional modules: enzymatic hydrolysis module for cell wall breakdown, grinding module for mechanical size reduction, ultrasonic module for cellular disruption, centrifugation module for solid-liquid separation, ceramic membrane ultrafiltration module for polysaccharide separation, macroporous adsorption resin module for polyphenol removal, spiral-wound membrane ultrafiltration module for SOD separation, and reverse osmosis module for Vc concentration. Each module handles a specific extraction task, making the overall complex process manageable and systematic.
Solution Approach 2:
Several intermediary substances and media are used to facilitate different extraction stages: enzymatic hydrolysis solution (cellulase, pectinase) as chemical mediator for cell wall degradation, grinding slurry as mechanical intermediary for size reduction, ultrasonic waves as physical intermediary for cellular disruption, centrifugal force as mechanical intermediary for separation, ceramic membrane as physical barrier for polysaccharide filtration, macroporous adsorption resin as chemical intermediary for polyphenol adsorption, spiral-wound membrane as physical barrier for SOD separation, and reverse osmosis membrane as physical barrier for Vc concentration. These intermediaries enable selective extraction at each stage.
3Productivity
If enzymatic hydrolysis and ultrasonic treatment are applied to break cell walls, then the release of active ingredients is improved, but the energy consumption and processing time increase
Solution Approach 1:
Enzymatic hydrolysis is applied as a preliminary treatment before mechanical grinding and ultrasonic treatment. The enzymatic breakdown of cell walls (cellulase and pectinase acting on cellulosic and pectic substances) pre-fragments the cellular structure, making subsequent mechanical and ultrasonic treatments more effective and requiring less energy. This preliminary chemical action reduces the intensity and duration needed for later physical treatment stages.
Solution Approach 2:
Enzymatic hydrolysis replaces purely mechanical methods of cell wall breakdown. Instead of using only high-pressure grinding or intense ultrasonic vibration to rupture cell walls, the patent employs biological enzymes (cellulase for cellulosic substances, pectinase for pectic substances) to chemically degrade cell wall components. This substitution reduces the energy input required for mechanical disruption while achieving comparable or superior cell wall breakdown efficiency.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method effectively extracts high-value polysaccharides, polyphenols, SOD, and Vc with high yield and purity, promoting the utilization of Rosa roxburghii Tratt pomace and reducing environmental impact, while producing powders suitable for industrial production with improved nutritional value.
Implementation Method 1
performing enzymatic hydrolysis treatment on the Rosa roxburghii Tratt pomace to obtain an enzymatic hydrolysis product
Implementation Method 2
performing enzymatic hydrolysis treatment on the Rosa roxburghii Tratt pomace
Implementation Method 3
performing ultrasonic treatment on the grinding slurry to obtain an ultrasonic treatment product
Implementation Method 4
performing centrifugation treatment on the ultrasonic treatment product and collecting the supernatant
Implementation Method 5
performing, using a ceramic membrane, ultrafiltration treatment on the supernatant to obtain a first permeate and a first retentate, the first retentate containing the polysaccharides
Implementation Method 6
treating the first permeate with a macroporous adsorption resin to obtain a deastringent liquid of Rosa roxburghii Tratt
Implementation Method 7
performing, using ethanol solution, elution treatment on the used macroporous adsorption resin, and evaporating and concentrating the eluate
Implementation Method 8
performing, using a spiral-wound membrane, ultrafiltration treatment on the deastringent liquid of Rosa roxburghii Tratt to obtain a second permeate and a second retentate, the second retentate containing the SOD
Implementation Method 9
performing reverse osmosis treatment on the second permeate to collect a third retentate, the third retentate containing the Vc
Data Source
AI summary
Provided are a method and system for simultaneously extracting polysaccharides of Rosa roxburghii Tratt, polyphenols of Rosa roxburghii Tratt, superoxide dismutase (SOD) of Rosa roxburghii Tratt, and Vc from Rosa roxburghii Tratt pomace. The method includes enzymatic hydrolysis treatment, grinding treatment, ultrasonic treatment, centrifugation treatment, ceramic membrane ultrafiltration treatment, macroporous adsorption resin treatment, elution treatment, evaporation and concentration treatment, spiral-wound membrane ultrafiltration treatment, and reverse osmosis treatment.