Rose Plants with Synergistic Nucleotide Sequences for Powdery Mildew Resistance
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Solution Overview
Problem
Current methods for breeding powdery mildew-resistant rose plants lack consistency and effectiveness due to genetic heterogeneity and the complexity of resistance mechanisms, with existing studies showing varying results and limited agreement on quantitative trait loci (QTL) and gene involvement, and existing resistance genes often come with deleterious side effects.
Innovation Solution
The introduction of specific nucleotide sequences (SEQ ID No. 1 and SEQ ID No. 2) that, when combined, provide dominant and synergistic powdery mildew resistance in rose plants, effectively conferring strong resistance against Podosphaera pannosa, the major powdery mildew-causing pathogen, and can be used in various ploidy levels of rose genomes.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional breeding methods are used to develop powdery mildew-resistant rose varieties, then resistance can be achieved, but the resistance is inconsistent and lacks effectiveness due to genetic heterogeneity and complex resistance mechanisms
Solution Approach 1:
The invention segments the complex resistance trait into two specific nucleotide sequences (SEQ ID NO: 1 and SEQ ID NO: 2) that can be individually identified and combined. This segmentation allows breeders to work with discrete genetic markers rather than dealing with the entire complex resistance mechanism, thereby improving consistency while managing genetic complexity.
Solution Approach 2:
The invention changes the parameter of resistance detection from phenotypic observation to genotypic identification using specific nucleotide sequences. This parameter change from observing disease resistance symptoms to detecting specific DNA sequences enables more reliable and consistent selection of resistant plants, overcoming the inconsistency of conventional breeding methods.
2Reliability
If existing resistance genes are introduced into rose plants, then some level of resistance is achieved, but intermediate phenotypes occur and resistance is not dominant
Solution Approach 1:
The invention merges two specific nucleotide sequences (SEQ ID NO: 1 and SEQ ID NO: 2) into a single genetic combination that produces dominant resistance. The synergistic interaction between these two sequences eliminates intermediate phenotypes and ensures consistent resistant expression, achieving both reliability and phenotype precision simultaneously.
3Object-affected harmful factors
If fungicides are used to control powdery mildew, then disease symptoms are reduced, but the control method becomes expensive, laborious and damaging to the environment
Solution Approach 1:
The invention applies preliminary action by identifying and selecting plants with specific resistance genes (SEQ ID NO: 1 and SEQ ID NO: 2) before disease outbreaks occur. This preventive genetic approach eliminates the need for reactive fungicide applications, thereby controlling disease while avoiding environmental damage from chemical treatments.
4Loss of information
If quantitative trait loci (QTL) mapping is performed to identify resistance genes, then genetic basis is revealed, but results vary across studies and agreement on specific QTL and genes is limited
Solution Approach 1:
The invention extracts the essential resistance information from complex QTL mapping studies by identifying two specific nucleotide sequences (SEQ ID NO: 1 and SEQ ID NO: 2) that are directly responsible for dominant resistance. This extraction consolidates varying study results into specific, actionable genetic markers, reducing information loss while simplifying the complexity of resistance gene identification.
Data Source
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AI summary
The present invention relates to rose plants such as cut roses, garden roses and pot roses comprising at least two genes providing resistance to a pathogen causing powdery mildew. Specifically, the present invention relates to rose plants resistant to the powdery mildew causing pathogen Podosphaera pannosa also known as Sphaerotheca pannosa var. rosae. The present invention further relates to methods for selecting the present powdery mildew rose plants. The present rose plants are characterized by comprising in their nuclear genome at least one nucleotide sequence represented by SEQ ID No. 1 and at least one nucleotide sequence represented by SEQ ID No. 2 wherein the combined presence of SEQ ID No. 1 and SEQ ID No. 2 provides powdery mildew resistance.