Rotatable Chamber Thrombin Isolation via Centrifugal Filtration
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Current methods for isolating thrombin from whole blood or plasma are inefficient and require improvement for enhanced clotting and healing processes.
Innovation Solution
A device and method utilizing a rotatable chamber with glass beads and polyacrylimide beads, along with a filter, to separate and extract thrombin by mixing and rotating the contents at specific speeds, allowing thrombin to pass through while retaining other components, thereby concentrating thrombin for enhanced clotting.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If current methods are used to isolate thrombin from whole blood or plasma, then the process is simple to perform, but the isolation efficiency is low and thrombin concentration is insufficient
Solution Approach 1:
The device segments the thrombin isolation process into distinct functional zones: a mixing chamber for reagent addition and initial mixing, and a centrifugal separation chamber for thrombin concentration. The chamber is divided by an angled barrier that creates separate flow paths for different components, enabling efficient separation while maintaining a relatively simple overall device structure.
Solution Approach 2:
The device utilizes dynamic centrifugal force generated by rotation to separate thrombin from other blood components. By rotating the chamber at controlled speeds, the system dynamically adjusts the separation efficiency, allowing thrombin to be concentrated in the centrifugal field while other components remain in the supernatant, thereby improving isolation efficiency without requiring complex mechanical structures.
2Quantity of substance
If a filter is used to separate thrombin from other components, then thrombin concentration increases, but the device structure becomes more complex
Solution Approach 1:
The device incorporates a porous filter within the centrifugal separation chamber that allows selective passage of thrombin while retaining larger blood components. The porous structure enables efficient filtration and concentration of thrombin during centrifugal rotation, achieving high thrombin concentration without requiring multiple filtration stages or complex membrane systems.
Solution Approach 2:
The angled barrier within the chamber acts as an intermediary element that directs fluid flow and facilitates separation. It creates a transition zone between the mixing and separation regions, guiding the plasma-thrombin mixture through the filter while maintaining structural simplicity. This intermediary structure enables efficient separation without adding significant device complexity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The method achieves a six to seven times concentration of thrombin, facilitating rapid wound clotting and healing by isolating thrombin effectively from whole blood or plasma, which can be used directly or in hemostatic agents.
Implementation Method 1
At least a portion of the sidewall is defined by a filter that permits passage of a first component of the multi-component composition out of the chamber through the filter and to the housing base. The filter restricts passage of a second component of the multi-component composition through the filter.
Implementation Method 2
rotating the chamber at a first speed for a first time period to mix the contents of the chamber and form a mixture
Implementation Method 3
rotating the chamber at a second speed for a second time period to cause thrombin to separate from the mixture and pass through a filter of the chamber and collect outside of the chamber
Data Source
AI summary
A device for isolating a component of a multi-component composition. The device includes a housing, a chamber, and a withdrawal port. The chamber is rotatably mounted within the housing. The chamber includes a chamber base and a sidewall. The side wall extends from the chamber base. At least a portion of the sidewall is defined by a filter that permits passage of a first component of the multi-component composition out of the chamber through the filter and to the housing base. The filter restricts passage of a second component of the multi-component composition through the filter. The withdrawal port extends from a position proximate to the housing base to an exterior of the device. The withdrawal port permits the withdrawal of the first component from the housing base to an exterior of the device.