Rotatable Sample Analysis Substrate With Magnet Chamber
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Solution Overview
Problem
Current sample analysis substrates face challenges in performing complex reaction steps, such as enzyme reactions and immune reactions, efficiently and accurately, particularly in separating bound and free components, which leads to measurement errors and inefficiencies.
Innovation Solution
A sample analysis substrate with a rotatable design, featuring a rotation shaft, holding chambers, reaction chambers, flow paths, and a magnet accommodation chamber, allows for precise transfer and separation of liquids and magnetic particles, enabling more complex reaction steps like sandwich immunoassays through controlled rotation and centrifugal forces.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If a conventional sample analysis substrate is used, then simple liquid transfer and mixing can be achieved, but complex reaction steps such as enzyme reactions and immune reactions cannot be performed efficiently
Solution Approach 1:
The substrate is divided into multiple functional chambers including a first holding chamber, reaction chamber, main chamber, and magnet accommodation chamber. Each chamber serves a specific function in the analysis process, enabling complex multi-step reactions while maintaining clear separation of operations.
Solution Approach 2:
The magnet accommodation chamber is positioned to accommodate a magnet that can capture magnetic particles within the main chamber. This nested arrangement allows the magnet to be housed within the substrate structure while still performing its function of separating bound and free components during immune reactions.
2Reliability
If liquid transfer is performed without precise control, then transfer speed is fast, but incorrect liquid transfers occur leading to measurement errors
Solution Approach 1:
The substrate is designed to be rotatable about a rotation shaft, allowing dynamic control of liquid transfer between chambers. By rotating the substrate to specific positions, liquids can be precisely transferred from the first holding chamber to the reaction chamber, and subsequently to the main chamber, ensuring accurate mixing and separation operations.
Solution Approach 2:
Flow paths with controlled openings serve as intermediaries between chambers. The first flow path connects the first holding chamber and reaction chamber, while the second flow path connects the reaction chamber and main chamber. These flow paths enable controlled liquid transfer and mixing, ensuring reliable analysis results.
3Measurement precision
If magnetic particles are not properly separated, then analysis process is simple, but bound and free components cannot be distinguished leading to measurement errors
Solution Approach 1:
A magnet is introduced as an intermediary component within the magnet accommodation chamber to capture magnetic particles in the main chamber. This allows for effective separation of bound and free components during sandwich immunoassays, enabling accurate measurement of analyte concentrations.
Solution Approach 2:
The magnet accommodation chamber is positioned at a specific location within the substrate to optimize magnetic particle capture. The chamber is designed with specific geometric characteristics that enhance the local magnetic field effect, improving separation efficiency for distinguishing bound and free components.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This design enables accurate and efficient analysis by preventing incorrect liquid transfers, ensuring precise washing and separation of reaction components, thereby enhancing measurement accuracy and simplifying the analysis process.
Implementation Method 1
a magnet accommodation chamber (120) located in the substrate, the magnet accommodation chamber being capable of accommodating a magnet (121); wherein the magnet (121) is located at a position at which the magnet (121) captures the magnetic particles (311) in the main chamber (107)
Implementation Method 2
A sample analysis substrate rotatable to transfer a liquid according to this disclosure includes a substrate including a rotation shaft
Data Source
AI summary
A sample analysis substrate includes a substrate; a first holding chamber; a reaction chamber; a first flow path having a first opening and a second opening respectively connected with the first holding chamber and reaction chamber; a main chamber; a second flow path having a third opening and a fourth opening respectively connected with the reaction chamber and the main chamber; and a magnet accommodation chamber capable of accommodating a magnet. The first opening is located closer to a rotation shaft than the second opening. The second opening is located closer to the rotation shaft than the third opening. The magnet accommodation chamber is located at a position at which, in the case where the magnet is accommodated in the magnet accommodation chamber, the magnet captures magnetic particles in the main chamber. The sample analysis substrate is rotatable to transfer a liquid.