Rotatable Upper-Cup Canister Assembly for Cryopreservation Retrieval

The patent implements a nested canister structure where multiple canisters (first canister and second canister) are arranged vertically within a single cryopreservation container. The first canister is positioned above the second canister, both sharing the same longitudinal axis. This nesting arrangement maximizes the use of vertical space in the container, significantly increasing storage capacity without requiring additional containers or complex horizontal expansion.

The canister assembly is segmented into multiple independent canisters (first canister with first lid, second canister with second lid) that can be independently accessed and manipulated. Each canister has its own lid that can be opened separately, allowing selective retrieval of samples from specific canisters without disturbing others, thus maintaining operational simplicity despite increased storage capacity.

The patent extracts only the necessary component (the second canister containing the required sample) from the cryopreservation container for retrieval, while the first canister remains in place. This selective extraction minimizes the volume of liquid nitrogen that needs to be displaced and reduces evaporation losses compared to removing an entire single canister or the complete assembly.

The canisters are pre-arranged in a vertical nested configuration with the second canister positioned below the first canister. This preliminary arrangement allows the retrieval system to access the second canister directly through the bottom of the first canister without requiring complete removal of the assembly, enabling sample retrieval while maintaining the protective liquid nitrogen environment for other samples.

The patent enables extraction of the second canister from the cryopreservation container while the first canister remains submerged in liquid nitrogen. This selective extraction allows the retrieved second canister to be quickly processed for sample access, minimizing the time that reproductive samples are exposed to warmer temperatures outside the cryopreservation environment.

The nested vertical arrangement with the second canister positioned at the bottom allows for pre-planned retrieval operations where the second canister can be accessed through the first canister's bottom opening. This preliminary structural arrangement facilitates rapid sample retrieval with minimal exposure time to ambient temperatures, protecting the integrity of temperature-sensitive reproductive samples.