Rotating Analyte Array Scanning for Faster Probe Detection
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Solution Overview
Problem
Biological sample processing systems and methods are often time-intensive and wasteful of valuable resources, necessitating more efficient methods and systems for sample processing and analysis.
Innovation Solution
A method involving the rotation of an open substrate with immobilized analytes, delivery of a solution via centrifugal force, and detection of bound probes using continuous rotational area scanning, compensated for velocity differences with anamorphic magnification gradients and optical imaging systems.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If conventional biological sample processing systems are used, then analysis can be performed, but the process is time-intensive and inefficient
Solution Approach 1:
The substrate is pre-loaded with multiple analytes in an array format before the analysis begins. This preliminary preparation allows for simultaneous processing of multiple samples, eliminating the need to process them sequentially and significantly reducing total processing time while maintaining high productivity
Solution Approach 2:
The substrate is divided into multiple discrete locations, each containing a different analyte. This segmentation enables parallel processing of multiple analytes in a single analysis run, improving productivity by processing multiple samples simultaneously rather than sequentially
2Productivity
If conventional biological sample processing systems are used, then analysis can be performed, but valuable resources such as reagents are wasted
Solution Approach 1:
Reagents are delivered to specific regions of the substrate where analytes are located, rather than flooding the entire substrate or using excessive amounts. This targeted delivery ensures that reagents are used efficiently and only where needed, reducing waste while maintaining high processing efficiency
Solution Approach 2:
The system is designed to deliver reagents precisely to the locations where they are needed on the substrate, eliminating the need for excess reagent to ensure coverage. The controlled delivery mechanism ensures reagents are used efficiently, reducing waste while maintaining productivity
3Measurement precision
If continuous rotational area scanning is used to detect bound probes, then detection accuracy is improved, but velocity differences at different radial positions must be compensated
Solution Approach 1:
The scanning system applies different scan rates to different radial positions on the substrate. Regions closer to the center of rotation use one scan rate while outer regions use a different scan rate, optimizing detection accuracy for each local area while accounting for the velocity differences caused by rotation
Solution Approach 2:
The scan rate parameter is dynamically adjusted based on the radial position being scanned. By changing the scan rate parameter according to the local velocity conditions at different radial positions, the system maintains detection accuracy across the entire substrate without requiring overly complex hardware modifications
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enhances the efficiency of analyte detection and analysis by compensating for velocity differences and optimizing resource use, thereby improving the speed and accuracy of biological sample processing.
Implementation Method 1
dispersing the solution across the open substrate at least by centrifugal force such that at least one of the plurality of probes binds to at least one of the immobilized analytes
Data Source
AI summary
Provided are systems and methods for analyte detection and analysis. A system can comprise an open substrate configured to rotate. The open substrate can comprise an array of immobilized analytes. A solution comprising a plurality of probes may be directed, via centrifugal force, across the array during rotation of the substrate, to couple at least one of the plurality of probes with at least one of the analytes to form a bound probe. A detector can be configured to detect a signal from the bound probe via continuous rotational area scanning of the substrate.