Rotating Body Cell Mass Aggregation via Centripetal Force
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Solution Overview
Problem
Existing cell culture devices fail to aggregate cell masses at a predetermined position in specific gravity adjustment solutions, despite creating a microgravity environment for cell growth.
Innovation Solution
A method and device utilizing a rotating body with a specific gravity adjustment solution and cell masses of lower specific gravity, where the rotating body is rotated to create centripetal force, allowing cell masses to aggregate around a central axis or base material, while ensuring biocompatibility and supplying nutrients through a liquid culture medium to prevent necrosis.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If a microgravity environment is created using rotating culturing devices, then cell masses can proliferate and extend in all directions, but the cell masses cannot be aggregated at a predetermined position
Solution Approach 1:
The patent applies local quality by creating different functional zones within the rotating body: a first rotating region for cell proliferation and a second rotating region for cell aggregation. By varying rotation speeds and directions in different spatial locations, the system achieves both omnidirectional growth and precise positional control of cell masses at predetermined aggregation points.
Solution Approach 2:
The patent utilizes dynamics by implementing variable rotation speeds and directions in different regions of the rotating body. The first rotating region rotates at a different speed than the second rotating region, allowing dynamic control over cell mass behavior - enabling both free proliferation and controlled aggregation at specific positions through temporal and spatial variation in rotational parameters.
2Stability of the object's composition
If cell masses are dispersed in specific gravity adjustment solution, then uniform suspension is achieved, but aggregation at predetermined position becomes difficult
Solution Approach 1:
The patent applies dynamics by varying rotation speeds between different regions - the first rotating region maintains slower rotation for uniform suspension and proliferation, while the second rotating region increases rotation speed to generate sufficient centrifugal force for precise aggregation at predetermined positions, thus dynamically transitioning from suspension to aggregation states.
Solution Approach 2:
The patent utilizes parameter changes by adjusting rotation speed as a controllable variable. By changing the rotation speed parameter in the second rotating region compared to the first region, the system transforms the physical state of cell masses from dispersed suspension to concentrated aggregation, enabling precise positional control while maintaining biocompatibility through controlled parameter variation.
3Productivity
If rotation speed is increased to aggregate cell masses, then aggregation efficiency improves, but cell viability may deteriorate due to excessive centrifugal force
Solution Approach 1:
The patent applies segmentation by dividing the rotating body into distinct rotating regions with different rotation speeds. The first rotating region operates at lower speeds to maintain cell viability during proliferation, while the second rotating region operates at higher speeds optimized for aggregation efficiency. This spatial segmentation of functional requirements allows both high aggregation efficiency and maintained cell viability to coexist.
Solution Approach 2:
The patent utilizes local quality by assigning different rotational characteristics to different spatial regions: the first rotating region provides gentle conditions for cell survival and growth, while the second rotating region provides intensified conditions for efficient aggregation. This localized optimization of rotation parameters ensures that high aggregation efficiency is achieved without compromising overall cell viability.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Effectively aggregates cell masses at a predetermined position within the specific gravity adjustment solution, maintaining their viability and preventing disturbances from air, thus enabling stable aggregation.
Implementation Method 1
the cell masses move to a predetermined position in the specific gravity adjustment liquid with respect to the specific gravity adjustment liquid due to the centripetal force that arises from the difference between the specific gravity of the specific gravity adjustment liquid and the specific gravity of the cell masses
Implementation Method 2
the specific gravity adjustment solution having biocompatibility, the cell masses having a lower specific gravity than the specific gravity adjustment solution
Data Source
AI summary
A method for aggregating cell masses includes performing a cell mass aggregating step of rotating a rotating body containing a specific gravity adjustment solution and cell masses to aggregate the cell masses, the specific gravity adjustment solution having biocompatibility, the cell masses having a lower specific gravity than the specific gravity adjustment solution.