Rotating Cell Culture System for Uniform Adherent Cell Growth
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Solution Overview
Problem
Current methods for large-scale cell culture are inadequate for efficiently culturing adherent cells, as they require separate processes and struggle to maintain high cell density and uniformity.
Innovation Solution
A large-scale cell culture system featuring an incubator with a cell culture part containing multiple plate-shaped supporters, a medium supply part, and a pump for circulating medium, maintaining constant temperature and carbon dioxide levels, and allowing for rotation of the cell culture part to ensure even cell attachment and nutrient distribution.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Productivity
If multiple supporters are arranged vertically in the cell culture part, then the cell culture area is increased and productivity is improved, but the medium circulation and nutrient distribution become uneven
Solution Approach 1:
The cell culture part is designed to rotate around a vertical axis, transforming the static arrangement of supporters into a dynamic system. This rotation ensures that all supporters receive uniform medium circulation and nutrient distribution, solving the unevenness problem while maintaining high cell culture area through vertical stacking
Solution Approach 2:
Multiple plate-shaped supporters are arranged vertically one above another within the same cell culture part, similar to nested dolls. This space-efficient arrangement maximizes the cell culture area within a compact volume, improving productivity without significantly increasing the device footprint
2Manufacturing precision
If the cell culture part is rotated, then the uniformity of cell attachment and nutrient distribution is improved, but the device complexity increases
Solution Approach 1:
The rotating cell culture part serves multiple functions: it enables uniform medium circulation to all supporters, facilitates even cell attachment across all plates, and allows for simplified harvesting by rotating to a convenient position. This multi-functionality justifies the added mechanical complexity
Solution Approach 2:
The rotation mechanism is integrated with the medium circulation system and support structure into a single unified assembly. The motor, supporters, and circulation channels are combined into one rotating unit, reducing the number of separate components and simplifying the overall device architecture
3Productivity
If a large number of cells are cultured simultaneously, then productivity is improved, but maintaining optimal cultural conditions becomes more difficult
Solution Approach 1:
The medium circulation system operates continuously through the rotating cell culture part, ensuring that all supporters receive fresh medium and nutrients without interruption. This continuous action maintains optimal cultural conditions across all cell cultures simultaneously, supporting high productivity with reliable condition control
Solution Approach 2:
The system incorporates sensors to monitor temperature, humidity, and medium quality, with control mechanisms that adjust conditions in real-time based on feedback from the culture environment. This ensures stable cultural conditions are maintained even when culturing large numbers of cells across multiple supporters
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This system enables the simultaneous cultivation of a large number of adherent cells with improved productivity, reduced costs, and uniform quality by maintaining optimal cultural conditions and efficient nutrient distribution.
Implementation Method 1
a pump that is disposed in the inner space, and is connected to the cell culture part and the medium supply part through a connection pipe, respectively, and circulates the medium so that the medium stored in the medium supply part is recovered to the medium supply part after being supplied to the cell culture part
Implementation Method 2
an incubator that includes an inner space to provides a culture environment in which a cell is stably cultured
Implementation Method 3
the supporter may include a plate-shaped nanofiber membrane that is coated with a protein motif
Data Source
AI summary
A large-scale cell culture system is provided. The large-scale cell culture system according to one embodiment of the present invention includes: an incubator that includes an inner space to provides a culture environment in which a cell is stably cultured; a cell culture part that is disposed in the inner space and includes multiple supporters for cell culture disposed therein; a medium supply part that is disposed in the inner space and stores a predetermined amount of medium supplied to the cell culture part; and a pump that is disposed in the inner space, and is connected to the cell culture part and the medium supply part through a connection pipe, respectively, and circulates the medium so that the medium stored in the medium supply part is recovered to the medium supply part after being supplied to the cell culture part, the multiple supporters being provided in a plate shape having a predetermined area, and arranged in a state separated apart from each other at a predetermined distance along a height direction inside the cell culture part.