Rotating Receptacle Cell Sorter for Millimeter-Scale Samples
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Solution Overview
Problem
Current cell sorters are inadequate for handling and sorting samples with diameters in the millimeter range, such as Xenopus oocytes and Zebrafish embryos, as they lack storage capabilities and controllable sample-removal mechanisms, leading to laborious manual sorting and limited throughput.
Innovation Solution
A sample sorter system with a receptacle that rotates to convey samples along a closed path, allowing for sorting and storage of samples up to 2000 μm in diameter, using sensors for characterization and selective removal without fluorescence markers, and a piping system for controlled delivery to subsequent apparatuses.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Extent of automation
If conventional cell sorters are used for sorting samples, then sorting capability is provided, but storage capability and controllable sample-removal mechanism are lacking, leading to manual sorting requirement
Solution Approach 1:
The patent combines the sorting function with storage capability in a single automated system. The sorting apparatus integrates a sorting mechanism that can direct samples into storage positions, eliminating the need for separate manual sorting operations and providing both sorting and storage automation in one device.
Solution Approach 2:
The sorting apparatus is designed to perform multiple functions including sorting, storing, and controlled sample removal. The system can operate in different modes (sorting mode, storage mode, and sample retrieval mode) using the same basic apparatus, reducing the need for multiple separate devices and manual interventions.
2Adaptability or versatility
If conventional cell sorters are used, then sorting of smaller samples is achieved, but handling of millimeter-range samples (up to 2000 μm) is inadequate
Solution Approach 1:
The patent modifies the operational parameters of the sorting apparatus to accommodate larger sample sizes. The aperture sizes, flow rates, and detection parameters are adjusted to handle samples up to 2000 μm in diameter, allowing the same apparatus to sort both small and large samples effectively.
3Measurement precision
If fluorescence markers are used for sample characterization, then detection capability is improved, but fluorescence damage occurs
Solution Approach 1:
The patent extracts the reliance on fluorescence markers for sample detection and characterization. By using alternative detection methods such as light scattering, absorption, or other non-fluorescent optical properties, the system achieves accurate sample characterization without exposing samples to potentially damaging fluorescence excitation.
4Productivity
If electrostatic charges are applied for sample sorting, then sorting efficiency is improved, but charge loss or damage occurs
Solution Approach 1:
The patent replaces or supplements the electrostatic sorting mechanism with an alternative approach that maintains sorting efficiency without relying on electrostatic charges. This could involve using magnetic fields, acoustic fields, or modified fluid dynamics to achieve sample separation without charge application and subsequent charge loss.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Enables efficient sorting and storage of various sample types, including opaque samples, with improved throughput and reduced manual labor, allowing for selective delivery to further treatment or analysis without damaging fluorescence or electrostatic charges.
Implementation Method 1
a drive which, when actuated, causes rotation of at least one circular component such that a flow regime is developed in the receptacle so that the samples suspended in the fluid are conveyable along a closed path
Implementation Method 2
at least one sensor which is adapted to determine characteristics of samples passing the examination site
Data Source
AI summary
The present invention discloses a sample sorter system adapted to receive and sort samples according to predetermined criteria. The sample sorter system comprises a receptacle that is adapted to receive and retain fluid and samples. The receptacle is operatively coupled with a drive and with a power source such that actuation of the drive causes the rotation of at least one circular component, which in turn causes the development of a flow regime in the receptacle such that the samples suspended in the fluid are conveyable along a closed path to at least one sample handling site that are positioned along said closed path.