Rotating Tray Cryopreservation Device for Uniform Pre-cooling
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Solution Overview
Problem
The current liquid nitrogen vapor fumigation freezing method for semen cryopreservation results in uneven vaporization and a risk of semen storage tubes detaching from aluminum frames, leading to suboptimal pre-cooling effects and the possibility of tubes falling into liquid nitrogen.
Innovation Solution
A low-temperature semen cryopreservation device featuring a tray with pivotally connected limiting parts and a tilting mechanism to ensure uniform fumigation, combined with a box lined with low thermal conductivity materials to conserve liquid nitrogen and prevent evaporation, allowing for efficient and secure freezing of multiple tubes.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Speed
If liquid nitrogen vapor fumigation freezing is used, then pre-cooling speed is improved, but uniformity of fumigation deteriorates
Solution Approach 1:
The tray is designed to be rotatable within the freezing chamber, allowing dynamic adjustment of tube orientation during fumigation. This enables uniform exposure of all tube surfaces to liquid nitrogen vapor, resolving the uneven fumigation issue while maintaining fast pre-cooling speeds.
Solution Approach 2:
The invention introduces rotational movement as a new dimension of action, transforming the static fumigation process into a dynamic three-dimensional exposure system. Tubes are exposed to vapor from multiple angles through rotation, achieving uniform fumigation while preserving the speed advantage of vapor phase freezing.
2Device complexity
If aluminum racks are used to hold tubes, then device complexity is reduced, but reliability of tube positioning deteriorates
Solution Approach 1:
The tray incorporates localized clamping structures at specific positions to secure tubes during rotation. These localized features provide reliable positioning without requiring complex overall structure, achieving both simplicity and reliability by applying constraint only where necessary.
Solution Approach 2:
The rotatable tray acts as an intermediary between the simple aluminum rack structure and the tubes, providing both support and rotational capability. This mediator enables reliable tube positioning and uniform fumigation while maintaining the simplicity of the overall device structure.
3Ease of operation
If tubes are clamped in fixed position, then ease of operation is improved, but uniformity of cooling deteriorates
Solution Approach 1:
The tray rotates automatically or manually during the freezing process, dynamically changing the orientation of tubes relative to the liquid nitrogen vapor source. This simple rotational mechanism ensures all tube surfaces receive uniform cooling while maintaining ease of operation through automated or straightforward manual rotation.
Solution Approach 2:
The rotational movement continues throughout the fumigation process, ensuring continuous and uniform exposure of all tube surfaces to cooling vapor. This continuous action maintains cooling uniformity without interrupting the freezing process or complicating operation.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The device ensures uniform pre-cooling of semen storage tubes, prevents them from falling, and optimizes liquid nitrogen usage, enhancing the freezing efficiency and safety compared to conventional methods.
Implementation Method 1
the inner wall and bottom end surface of the box are covered with low thermal conductivity material
Implementation Method 2
This method takes advantage of the low temperature provided by the evaporation of liquid nitrogen to pre-cool semen samples before immersing them in liquid nitrogen
Implementation Method 3
immersing them in liquid nitrogen for fumigation
Data Source
AI summary
This invention provides a low-temperature semen cryopreservation device, comprising: a box body for containing low-temperature cryoprotectant fluid; a tray, one end of which is pivotally connected to the inner side wall of the box body, and a limiting portion is provided on the tray for limiting the movement of the cryoprotectant tube. Two openings are sequentially arranged along the height direction on one side of the box body relative to the hinge end, with the height of one opening higher than that of the hinge end, and the height of the other opening lower than that of the hinge end; a limiting plate, which can be detachably inserted into either opening. When the limiting plate is switched from one opening to the other, the tray tilts to drive the cryopreservation tube to rotate 180 degrees, thereby ensuring the low-temperature cryoprotectant fluid to evenly fumigate the cryopreservation tube, improving the pre-cooling effect.