Roundworm Coproantigen Detection via Polyclonal Antibodies
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Solution Overview
Problem
Current methods for diagnosing roundworm infections in animals are time-consuming, require specialized equipment, and have low specificity, making it difficult to accurately identify roundworm infections without cross-reacting with other types of worms, and are limited by the need for serum samples which can be impractical to obtain.
Innovation Solution
Development of a device using polyclonal antibodies specific to roundworm antigens immobilized on a solid support, allowing for the detection of roundworm coproantigens in fecal samples without cross-reacting with hookworm, whipworm, or heartworm antigens, utilizing lateral flow immunoassay or ELISA techniques.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If microscopic examination methods are used for diagnosis, then adoption is high and equipment requirements are met, but time consumption increases, specificity decreases, and operator skill dependency increases
Solution Approach 1:
The patent replaces mechanical microscopic examination with an immunological detection system using antibody-antigen reactions. The device uses polyclonal antibodies immobilized on a solid support to detect roundworm coproantigens, substituting the mechanical microscopy process with a biochemical detection method that provides both speed and accuracy.
Solution Approach 2:
The patent introduces polyclonal antibodies as intermediaries between the fecal sample and the detection system. These antibodies specifically bind to roundworm coproantigens, serving as a mediator that enables specific detection without requiring direct microscopic observation, thereby reducing time consumption while maintaining or improving diagnostic accuracy.
2Reliability
If microscopic examination methods are used, then general parasitic worm detection is possible, but specificity for roundworm identification decreases
Solution Approach 1:
The patent applies local quality by using polyclonal antibodies that are specifically raised against roundworm coproantigens. These antibodies have localized specificity for roundworm antigens, allowing the device to distinguish roundworm infections from other parasitic worms even though the overall detection system remains relatively simple.
3Reliability
If serum samples are used for antigen detection, then detection capability is achieved, but sample obtainability decreases
Solution Approach 1:
The patent inverts the traditional approach by detecting coproantigens in fecal samples instead of detecting antigens in serum. This inversion allows for easier sample collection since feces can be obtained non-invasively, while maintaining detection capability through the use of specific polyclonal antibodies that recognize roundworm antigens in the fecal environment.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution enables specific detection of roundworm infections in fecal samples, reducing the need for serum samples and improving diagnostic accuracy by using polyclonal antibodies that bind specifically to roundworm antigens, even in samples with co-infections, and is applicable to various animal species.
Implementation Method 1
one or more polyclonal antibodies specific for one or more roundworm antigens are immobilized on the solid support
Data Source
AI summary
A composition, device, kit and method for detecting the presence or absence of roundworm in a fecal sample. The composition, device, kit and method of the present invention may be used to confirm the presence or absence of roundworm in a fecal sample from a mammal that may also be infected with one or more of hookworm, whipworm, and heartworm.