RT-PCR Amplification Curve Analysis Interface
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Solution Overview
Problem
Researchers face challenges in efficiently viewing and analyzing large numbers of amplification curves generated by RT-PCR instruments, as well as managing the maintenance and calibration of multiple biological instruments, due to the manual nature of data comparison and instrument tracking.
Innovation Solution
A graphical user interface is developed that allows users to easily select, view, and annotate amplification curves, with features like zooming, commenting, and tagging, as well as a system for monitoring instrument status and maintenance schedules, utilizing touch controls and pinch-to-zoom gestures for intuitive interaction.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If manual methods are used to compare and analyze biological data, then users can examine individual samples in detail, but the time required to analyze large numbers of samples increases significantly
Solution Approach 1:
The patent creates visual copies of biological data through graphical representations. Amplification curves are displayed as visual plots, and samples are represented as selectable items in a results table. This allows users to quickly scan and compare multiple samples visually rather than examining raw numerical data for each sample individually, significantly reducing analysis time while maintaining the ability to inspect details of selected samples.
Solution Approach 2:
The patent segments the large set of biological data into individual, selectable sample entries in a results table. Each sample can be independently selected and examined, allowing users to navigate through data in manageable units rather than being overwhelmed by the entire dataset at once. This segmentation enables efficient browsing and focused analysis of specific samples of interest.
2Productivity
If multiple biological instruments are operated simultaneously, then productivity increases, but tracking maintenance and calibration becomes more difficult
Solution Approach 1:
The patent merges the management of multiple biological instruments into a single integrated system. The results table consolidates data from multiple qPCR instruments, and the system provides unified access to instrument status, maintenance schedules, and calibration information. This consolidation allows researchers to manage multiple instruments through one interface rather than separately tracking each device, reducing management complexity while maintaining high productivity.
3Loss of information
If detailed information about each sample is displayed, then data completeness is improved, but the interface becomes cluttered and harder to navigate
Solution Approach 1:
The patent extracts detailed sample information from the main results table and presents it in a separate, dedicated panel. When a user selects a sample in the results table, the sample information panel displays comprehensive details including amplification curves, genotyping data, and other biological information. This extraction allows the main interface to remain clean and navigable while providing complete data access through the separate panel.
Solution Approach 2:
The patent transitions from a two-dimensional table view to a multi-dimensional display by adding graphical amplification curves and sample information panels. Selected samples are highlighted in the results table, and their corresponding amplification curves are displayed in a separate graphical region. This dimensional expansion allows comprehensive data presentation without cluttering the primary data table, improving both information completeness and interface usability.
Data Source
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AI summary
A method for identifying a reaction site associated with an amplification curve from a plurality of amplification curves (104, 204, 304, 404) is provided. The method includes receiving amplification data from a plurality of reaction sites, wherein each reaction site contains a sample. The method further includes generating a plurality of amplification curves (104, 204, 304, 404) from the amplification data and displaying a first portion of the plurality of amplification curves (104, 204, 304, 404) on a display screen (1212). The method includes displaying a list of indications of reaction sites (A24-A29) associated with the first portion of amplification curves (104, 204, 304, 404) alongside the first portion of amplification curves (104, 204, 304, 404) on the display screen (1212). Then, the method includes adjusting the view to display a second portion of the plurality of amplification curves (104, 204, 304, 404). The method further includes dynamically adjusting the list to display indications of reaction sites (A24-A29) associated with the second portion of amplification curves (104, 204, 304, 404) alongside the second portion of amplification curves (104, 204, 304, 404) on the display screen (1212), wherein the list is configured to be scrollable.