RT-PCR Primer-Probe Set for Foreign Tissue Detection in Meat
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Solution Overview
Problem
Current methods for detecting foreign tissues, such as offal, in meat and meat products are inefficient, subjective, and require expert personnel, making them unsuitable for objective and precise analysis.
Innovation Solution
An analysis method using molecular biology and genetic techniques based on mRNA and expressed sequence tags (EST) to detect foreign tissues through reverse-transcriptase polymerase chain reaction (RT-PCR) and Real-Time PCR analysis, employing specific primer-probe sets to identify offal and other foreign tissues.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If histological analysis is used to detect foreign tissues, then detection capability is achieved, but the method requires expert personnel and produces subjective results
Solution Approach 1:
The patent replaces the mechanical/optical histological analysis system with a molecular biology-based RT-PCR system. Instead of using microscopes and expert visual inspection, the invention uses reverse transcription polymerase chain reaction to detect foreign tissues through amplification of species-specific DNA sequences, followed by automated analysis systems that eliminate subjective human judgment.
Solution Approach 2:
The patent introduces DNA as an intermediary substance for detection. Rather than directly examining tissue morphology under a microscope, the method extracts DNA from the sample, uses it as a template for PCR amplification, and detects the presence of foreign tissues through species-specific DNA sequence identification, thereby removing the need for expert visual assessment.
2Measurement precision
If histological analysis is used to detect foreign tissues, then detection capability is achieved, but the method cannot provide numerical output
Solution Approach 1:
The patent replaces the subjective visual assessment system with an objective molecular biology system that produces quantifiable results. The RT-PCR method generates numerical data through cycle threshold (Ct) values, amplification curves, and DNA concentration measurements, which can be automatically analyzed and compared against reference values to determine the presence and quantity of foreign tissues.
3Measurement precision
If molecular biology methods are used to detect foreign tissues, then objective and precise results are provided, but detection limit and sensitivity must be optimized
Solution Approach 1:
The patent optimizes detection sensitivity by adjusting critical PCR parameters including primer and probe concentrations, annealing temperatures, and cycle numbers. The method uses fluorescently labeled probes with specific emission wavelengths and optimizes the thermal cycling conditions to maximize amplification efficiency while maintaining specificity, thereby achieving a detection limit of 1% foreign tissue in meat products.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This method provides objective and precise detection of foreign tissues, including offal, in meat and meat products, with a detection limit of up to 1% and the ability to differentiate between various animal species, even after heat treatment.
Implementation Method 1
Conversion of isolated RNA into cDNA by reverse transcriptase PCR reaction
Implementation Method 2
Amplification of cDNAs by using RT-PCR method with a primer-probe set
Data Source
AI summary
The present invention relates to an analysis method in which method for molecular detection is used based on an expressed sequence tag (EST - Expressed Sequence Tag) having mRNA and / or expression as the mediator molecule in the formation of protein structures providing the specificity of each tissue and organ for the detection of foreign tissue or tissues, such as offal, which may be found in meat and meat products other than carcass meat and relates to a primer-probe set used in an analysis method.