Saccharide-Coated Solid-Phase Carrier for Specific Capture
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Solution Overview
Problem
Existing solid-phase carriers for capturing target substances face challenges with high nonspecific adsorption of biological substances, which complicates the detection and purification of specific proteins, peptides, nucleic acids, and cells, and can reduce the activity of bound ligands.
Innovation Solution
A solid-phase carrier with a saccharide-coated base material and a linker of 5-100 atoms having a reactive functional group for binding ligands, designed to minimize nonspecific adsorption and maintain ligand activity, is developed. This carrier is produced by chemically binding a saccharide to a linker molecule and then to a base material, such as magnetic particles, creating a surface suitable for specific interactions.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If conventional affinity resins are used for capturing target substances, then the capture capability is achieved, but nonspecific adsorption of biological substances occurs which complicates detection and purification
Solution Approach 1:
The patent introduces a spacer arm as an intermediary component between the solid-phase carrier and the ligand. This spacer arm mediates the interaction by providing physical separation that prevents nonspecific adsorption of biological substances onto the carrier surface, while still allowing the ligand to maintain its specific binding capability to the target substance.
Solution Approach 2:
The patent applies different functional properties to different parts of the carrier structure. The solid-phase carrier surface is designed to be non-adsorptive (low nonspecific binding), while the ligand-bound region maintains high specific binding affinity. This spatial differentiation of functional qualities resolves the contradiction between specific capture and nonspecific adsorption.
2Reliability
If conventional solid-phase carriers are used, then target substance capture is possible, but the activity of bound ligands is reduced
Solution Approach 1:
The spacer arm serves as a protective intermediary that separates the ligand from the solid-phase carrier surface. This separation prevents the carrier surface from interacting with and potentially deactivating the ligand, thereby maintaining ligand activity while still enabling target capture function.
Solution Approach 2:
The patent extends the ligand-carrying structure into the third dimension by using a spacer arm that protrudes from the carrier surface. This dimensional extension places the ligand in a different spatial zone away from the carrier surface, reducing unwanted interactions and preserving ligand activity.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The solution significantly reduces nonspecific adsorption of biological substances, allowing for efficient capture and maintaining the activity of bound ligands, thereby enhancing the detection and purification of target substances.
Implementation Method 1
The presence of a nonspecific intermolecular interaction that prevents the selection and purification of a desired molecule based on a specific intermolecular interaction has raised a problem
Implementation Method 2
a linker of 5 to 100 atoms having a reactive functional group for binding a ligand that specifically binds to a target substance, in which the saccharide is chemically bound to the linker
Implementation Method 3
an attempt to capture a molecule that exhibits a specific interaction with a particular molecule using a technique based on intermolecular interactions
Data Source
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AI summary
To provide a solid-phase carrier that has extremely low nonspecific adsorption of biological substances such as proteins, peptides, nucleic acids and cells, and is capable of maintaining the activity of bound ligands at a high level; and a method for capturing a target substance using the solid-phase carrier. A method for capturing a target substance, including: a step for preparing a solid-phase carrier for capturing a target substance, the carrier having a base material, at least a portion of the surface thereof being formed oforcoatedwitha saccharide, and having a linker of 5-100 atoms having a reactive functional group for binding a ligand that specifically binds to the target substance, wherein the saccharide is chemically bound to the linker; a step for binding the ligand to the solid-phase carrier to obtain a solid-phase carrier having a ligand bound thereto; and a step for bringing the solid-phase carrier having the ligand bound thereto into contact with a sample containing the target substance that specifically binds to the ligand.