Salmonella Bacteriophage Composition for Broad Host Range Control

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Solution Overview

Problem

Existing bacteriophages used to control Salmonella bacteria face issues such as resistance development, narrow host range, and the need for effective control of multidrug-resistant strains like S. Typhimurium.

Innovation Solution

Isolation and characterization of novel bacteriophages with specific genomic sequences and protein compositions that exhibit broad host range and bacteriolytic activity against Salmonella species, including multidrug-resistant S. Typhimurium.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If a specific bacteriophage is used repeatedly to control Salmonella bacteria, then effective bacteriolytic activity is achieved, but resistance development in Salmonella strains occurs

Engineering Contradiction:

Improvebacteriolytic activityVSAvoidresistance development

Core Design Contradiction:

ReliabilityVSAdaptability or versatility

Solution Approach 1:

The patent applies universality by developing a bacteriophage composition containing multiple different bacteriophages (at least two distinct phages) that can collectively target multiple Salmonella strains. This multi-phage approach ensures broad-spectrum activity against various Salmonella serotypes while reducing the likelihood of resistance development, as bacteria would need to develop simultaneous resistance to multiple phages with different host ranges and mechanisms

Inventive Principle:

Principle #6Universality (Multi-functionality)

2Measurement precision

If a bacteriophage with narrow host specificity is used, then high target precision is achieved, but the range of applicable Salmonella strains is limited

Engineering Contradiction:

Improvetarget specificityVSAvoidhost range

Core Design Contradiction:

Measurement precisionVSAdaptability or versatility

Solution Approach 1:

The patent merges multiple bacteriophages with different host specificities into a single composition. Each phage maintains its specific targeting capability for particular Salmonella strains, while the combination provides expanded coverage across diverse Salmonella serotypes including S. Enteritidis, S. Typhimurium, and other multidrug-resistant strains

Inventive Principle:

Principle #5Merging (Combining)

3Productivity

If conventional small-molecule antimicrobial agents are used continuously, then initial control effect is achieved, but multidrug resistant bacteria emerge

Engineering Contradiction:

Improvecontrol effectivenessVSAvoidresistance emergence

Core Design Contradiction:

ProductivityVSReliability

Solution Approach 1:

The patent substitutes chemical antimicrobial agents with biological bacteriophages as the control mechanism. Bacteriophages are viruses that specifically infect and lyse bacteria through biological mechanisms (attachment to surface receptors, injection of genetic material, replication within host, and lysis), replacing the chemical action of small-molecule antibiotics with a targeted biological system that does not induce the same resistance mechanisms

Inventive Principle:

Principle #28Mechanics substitution (Replace mechanical system)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

Provides effective bacteriolytic agents capable of targeting a wide range of Salmonella strains, including resistant variants, offering a novel approach to control and identify Salmonella infections.

Implementation Method 1

Many phages attach to target host bacteria, then inject their own DNA into the bacteria, and self-amplify utilizing the translational mechanism of the bacteria

Methodology Applied

Scientific EffectViral infection and replication:

Implementation Method 2

Furthermore, the bacteria are bacteriolyzed, and consequently, the amplified phages are propagated, and an infection into new target bacteria is repeated

Methodology Applied

Scientific EffectBacteriolysis:

Data Source

PatentEP4692335A1Bacteriophage, bacteriolytic agent for bacteria belonging to genus salmonella, composition, and method for controlling bacteria belonging to genus salmonella

Publication Date: 2026.02.11 KANEKA CORP

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Figure 3A~3B

AI summary

An aim of the present disclosure is: (i) to provide a novel bacteriophage having bacteriolytic activity against bacteria of the genus Salmonella, such as S. Enteritidis, or a bacteriolytic agent consisting of the same; (ii) to provide a bacteriophage having a wide host range for bacteria of the genus Salmonella, or a bacteriolytic agent consisting of the same; (iii) to provide a host-specific bacteriophage or an effective Salmonella bacteriolytic agent consisting of the same; or (iv) provide a bacteriophage capable of effectively controlling S. Typhimurium, and in particular, multidrug-resistant S. Typhimurium, or a bacteriolytic agent consisting of the same. The present disclosure provides: a bacteriophage having a specific genomic DNA sequence; a Salmonella bacteriolytic agent consisting of the bacteriophage; and a composition comprising the same.