Salt-Free Miso Production via High-Pressure Fermentation

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Solution Overview

Problem

Conventional miso production is lengthy, and attempts to shorten the fermentation process often result in products with compromised taste and flavor, particularly when aiming for salt-free miso, which is also less effective as a hepatic function-improving and hypertension-ameliorating agent.

Innovation Solution

Reacting steamed soybeans with koji culture under applied heat and pressure (50-80 MPa, 50-60°C) for 1-5 days to produce a salt-free miso that maintains flavor and functionality, eliminating the need for added salt and reducing production time.

Engineering Contradictions & Design Principles

VSEngineering Contradiction Analysis

1Reliability

If conventional fermentation operations are carried out over an extended period of time at low temperature, then the koji is not deactivated and the fermentation is complete, but the production time is long and common salt or alcohol must be added to prevent microorganism growth

Engineering Contradiction:
Improvefermentation completionVSAvoidproduction time
Core Design Contradiction:
ReliabilityVSLoss of time

Solution Approach 1:

The invention changes the fermentation parameters by applying high pressure (50-80 MPa) and moderate temperature (50-60°C) to accelerate the fermentation process. This parameter change allows the fermentation to complete in 1-5 days instead of extended periods, while the high pressure prevents microorganism growth without requiring added salt or alcohol

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The invention uses high pressure as an intermediary factor to control the fermentation process. The applied pressure (50-80 MPa) serves as a mediator that accelerates enzymatic reactions while inhibiting unwanted microorganism growth, eliminating the need for conventional preservation methods like salt or alcohol addition

Inventive Principle:
Principle #24Intermediary (Mediator)

2Reliability

If salt is added to prevent microorganism growth during production, then the fermentation process is controlled, but the miso contains high salt content which is harmful for hypertension

Engineering Contradiction:
Improvefermentation controlVSAvoidsalt content
Core Design Contradiction:
ReliabilityVSObject-affected harmful factors

Solution Approach 1:

The invention uses high pressure (50-80 MPa) as an intermediary to achieve both fermentation control and hypertension prevention. This pressure condition inhibits microorganism growth without requiring salt addition, thereby controlling fermentation while eliminating the harmful salt content that would otherwise be needed for preservation

Inventive Principle:
Principle #24Intermediary (Mediator)

Solution Approach 2:

The invention converts the harmful effect of high pressure (which could damage soybeans) into a beneficial fermentation control mechanism. The applied pressure of 50-80 MPa and temperature of 50-60°C creates conditions that accelerate beneficial enzymatic reactions while inhibiting harmful microorganism growth, eliminating the need for salt

Inventive Principle:
Principle #22Blessing in disguise (Convert harm into benefit)

3Object-affected harmful factors

If salt-free miso is produced to reduce salt content for health benefits, then hypertension is improved, but the taste and flavor (soy flavor) are compromised

Engineering Contradiction:
Improvesalt contentVSAvoidtaste and flavor
Core Design Contradiction:
Object-affected harmful factorsVSEase of manufacture

Solution Approach 1:

The invention changes the fermentation parameters by applying high pressure (50-80 MPa) and moderate temperature (50-60°C) to accelerate the fermentation process. This parameter change allows the fermentation to complete in 1-5 days instead of extended periods, while the high pressure prevents microorganism growth without requiring added salt or alcohol

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The invention uses high pressure as an intermediary factor to control the fermentation process. The applied pressure (50-80 MPa) serves as a mediator that accelerates enzymatic reactions while inhibiting unwanted microorganism growth, eliminating the need for conventional preservation methods like salt or alcohol addition

Inventive Principle:
Principle #24Intermediary (Mediator)

4Productivity

If the fermentation step is shortened to reduce production time, then productivity is improved, but the taste and flavor are compromised

Engineering Contradiction:
Improveproduction speedVSAvoidtaste and flavor
Core Design Contradiction:
ProductivityVSEase of manufacture

Solution Approach 1:

The invention changes the fermentation parameters by applying high pressure (50-80 MPa) and moderate temperature (50-60°C) to accelerate the fermentation process. This parameter change allows the fermentation to complete in 1-5 days instead of extended periods, while the high pressure prevents microorganism growth without requiring added salt or alcohol

Inventive Principle:
Principle #35Parameter changes

Solution Approach 2:

The invention replaces the conventional time-based fermentation mechanism with a pressure-based acceleration system. By applying high pressure (50-80 MPa), the enzymatic reactions are accelerated mechanically, allowing the fermentation to complete much faster while maintaining quality, thus replacing the passive time-based process with an active pressure-driven process

Inventive Principle:
Principle #28Mechanics substitution (Replace mechanical system)

Applied Scientific Principles

This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.

Function Achieved in This Case

The method produces salt-free miso with enhanced hepatic function-improving and hypertension-ameliorating effects, offering a healthier alternative with improved taste and reduced production time, suitable for use in pharmaceutical products.

Implementation Method 1

fermenting steamed soybeans and koji culture under applied heat and pressure

Methodology Applied
Scientific EffectFermentation: Fermentation

Implementation Method 2

a pressure of 50 to 80 MPa and a temperature of 50 to 60°C

Methodology Applied
Scientific EffectPressure: Pressure Increase

Implementation Method 3

a temperature of 50 to 60°C

Methodology Applied
Scientific EffectHeating: Heating

Data Source

PatentEP3130233B1Salt-free miso production method, salt-free miso, hepatic function improvement agent, and hypertension improvement agent
Publication Date: 2020.02.19 MARUZEN PHARMA
  • EP3130233B1 patent drawingFigure 1~2
  • EP3130233B1 patent drawingFigure 3~4
  • EP3130233B1 patent drawingFigure 5~6

AI summary

Provided are: a production method for salt-free miso, in which steamed soy beans and koji are pressurized, heated, and fermented; salt-free miso obtained by said production method; and a hepatic function improvement agent and a hypertension improvement agent that contain said salt-free miso as an effective component thereof.