Same-Chromophore Compound Quantification Using One Standard
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Solution Overview
Problem
The existing single-standard quantification method for multiple components (SSDMC) faces challenges such as increased errors due to multiple calculation steps, fluctuating relative correction factors (RCFs), and difficulty in selecting internal reference substances, leading to inaccuracies and inefficiencies in complex sample analysis.
Innovation Solution
A method using a single standard with a chromophore to quantify multiple compounds by plotting standard curves based on mass and molar concentrations, eliminating the need for individual standard curves and RCFs, and relying on molar mass for accurate quantification.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If traditional multi-standard curve method is used for quantification, then measurement precision is improved, but device complexity and loss of time increase due to requiring multiple standards and individual standard curves for each component
Solution Approach 1:
The patent applies universality by using a single standard compound that can quantify multiple different compounds with the same chromophore. The standard curve established with one standard compound serves as a universal reference for determining concentrations of other compounds sharing the same chromophore, eliminating the need for separate standard curves for each component.
Solution Approach 2:
The patent merges the quantification of multiple components into a single standard curve system. Instead of requiring individual standard curves for each component, the method combines all quantifications under one unified standard reference, reducing the number of standards needed and simplifying the overall quantification process.
2Device complexity
If single-standard quantification method is used, then device complexity is reduced, but measurement precision deteriorates due to increased errors from multiple calculation steps and fluctuating RCFs
Solution Approach 1:
The patent changes the fundamental parameter of quantification from using multiple standards with individual curves to using a single standard with a unified curve. This parameter change eliminates the need for calculating relative correction factors (RCFs) for each component, as all components are quantified relative to the same standard reference, thereby reducing calculation steps and associated errors.
3Measurement precision
If multiple individual standard curves are established for each component, then measurement precision is improved, but loss of time increases due to extended method development time
Solution Approach 1:
The patent applies preliminary action by establishing a single standard curve using one standard compound before quantifying any sample. This pre-established standard curve serves as a universal reference for all subsequent quantifications of different compounds with the same chromophore, eliminating the need to develop and establish multiple separate standard curves during method development.
4Adaptability or versatility
If RCFs are calculated for each component, then adaptability is improved for different components, but measurement precision deteriorates due to large deviations from multiple influencing factors
Solution Approach 1:
The patent extracts and eliminates the need for calculating relative correction factors (RCFs) for each component. By using a single standard reference, the method removes the source of error associated with RCF calculations, which are influenced by multiple factors including instrument conditions, sample matrix effects, and standard purity, thereby improving measurement precision.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
Reduces errors, saves time and costs, and improves efficiency by using a single standard to determine multiple compounds with high accuracy, independent of external and internal factors affecting traditional SSDMC methods.
Implementation Method 1
detecting standard working solutions with different concentrations by high-performance liquid chromatography
Implementation Method 2
an absorbance ymeasured of the compound to be tested is the same as an absorbance yr of the standard
Data Source
AI summary
A method for quantifying a plurality of compounds with the same chromophore using a single standard is provided. In the method, the standard curve of the standard is plotted, the calculation curve of the compound to be tested with the same chromophore as the standard is derived, and the quantitative determination of the compound to be tested is realized according to the calculation curve. The method requires few standards, leads to accurate determination results, and also saves time and costs in a series of methodological investigations such as establishment of individual standard curve for each component, thereby improving the efficiency of method development.