Biological Sample Analysis Calibration for Uniform Photodetector Sensitivity
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Solution Overview
Problem
Existing biological sample analysis systems face challenges in accurately specifying particles with predetermined characteristics due to sensitivity differences among photodetectors, requiring multiple kinds of beads for adjustment and struggling with sensitivity calibration methods that fail to achieve consistent signal-to-noise ratios.
Innovation Solution
A biological sample analysis system and method that utilizes an information processing unit to process light intensity data, enabling the specification of particles with predetermined characteristics by adjusting photodetector sensitivity using sample beads and calibrating output levels with Vop, achieving consistent sensitivity across different photodetectors.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Adaptability or versatility
If multiple kinds of beads are used for adjustment and calibration, then the ability to perform various adjustments is improved, but the complexity of data acquisition and processing increases
Solution Approach 1:
The patent extracts and processes only the necessary data from the complex mixture of particles. By using gating techniques and selective data acquisition, the system isolates relevant information from the background noise and other particle types, reducing processing complexity while maintaining versatility.
Solution Approach 2:
The system uses a single multi-functional bead preparation method that can serve multiple purposes: calibration, adjustment, and characterization. This universal approach allows one sample to perform various functions that would traditionally require multiple separate bead preparations, improving adaptability without proportionally increasing complexity.
2Measurement precision
If sensitivity calibration is performed using conventional methods, then photodetector calibration is achieved, but consistent signal-to-noise ratios across different photodetectors cannot be maintained
Solution Approach 1:
The patent implements feedback mechanisms where the system continuously monitors and adjusts photodetector sensitivity based on real-time measurements from standardized beads. This feedback loop enables dynamic compensation for variations in signal-to-noise ratios, ensuring consistent performance across different photodetectors while maintaining calibration accuracy.
3Ease of manufacture
If digital adjustment methods are used for sensitivity calibration, then calibration processing is simplified, but approximation of signal-to-noise ratio is poor
Solution Approach 1:
The patent changes the fundamental parameter of adjustment from digital to analog domain. By performing sensitivity calibration in the analog domain using voltage application coefficients and control signals, the system achieves both simplified processing and accurate signal-to-noise ratio approximation, overcoming the limitations of purely digital methods.
4Measurement precision
If conventional calibration methods are used, then calibration can be performed, but sensitivity differences among photodetectors cannot be uniformly adjusted
Solution Approach 1:
The patent applies local quality adjustment by individually calibrating each photodetector with customized voltage application coefficients and control signals. Instead of a uniform calibration approach, the system tailors specific adjustment parameters to each photodetector's characteristics, achieving uniform sensitivity across all detectors while maintaining individual optimization.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
The system effectively specifies particles with predetermined characteristics, ensuring uniform sensitivity across apparatuses and maintaining consistent output levels, even with aging detectors, by employing an analog adjustment method that aligns sensitivity and signal-to-noise ratios.
Implementation Method 1
a detection unit that detects light generated by light irradiation to particles
Implementation Method 2
a particle group such as a cell, a microorganism, or a liposome is labeled with a fluorescent pigment, and the intensity and/or pattern of fluorescence generated from the fluorescent pigment excited by irradiating each particle of the particle group with light is measured
Data Source
AI summary
To provide a technique for specifying particles having predetermined characteristics from multiple kinds of particles having different characteristics.The present invention provides a biological sample analysis system including a detection unit that detects light generated by light irradiation to particles, and an information processing unit that processes light intensity data detected by the detection unit, in which the information processing unit executes processing of specifying particles having predetermined characteristics from multiple kinds of particles having different characteristics on the basis of the light intensity data generated by light irradiation to a sample containing particle groups including the multiple kinds of particles having different characteristics, and the like.