Sample Analyzer Coagulation Endpoint Detection
Find Innovative SolutionsGenerate Solutions
Solution Overview
Problem
Existing blood coagulation analyzers face challenges in accurately determining coagulation time due to noise in scattered light measurements and fluctuations, especially in samples with high fibrin or heparin concentrations, which complicates the determination of saturation values and coagulation reaction endpoints.
Innovation Solution
A sample analyzer that generates a reaction curve representing changes in optical information over time, determines areas before and after an evaluation target time to identify the reaction endpoint, and calculates coagulation time based on these areas, reducing noise impact and improving accuracy.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Measurement precision
If the saturation value is determined based on the difference between two input values, then the coagulation time can be calculated, but the determination accuracy deteriorates due to large fluctuations and noise in the difference values
Solution Approach 1:
The patent divides the scattered light data into multiple segments and calculates the average value across segments to determine the saturation value. This segmentation approach reduces the impact of noise and fluctuations in individual measurements, thereby improving determination accuracy while maintaining reliability.
Solution Approach 2:
The patent performs preliminary smoothing processing on the scattered light data before calculating the saturation value. By pre-processing the data to reduce noise and fluctuations, the subsequent saturation value determination becomes more accurate and reliable.
2Measurement precision
If the coagulation time is calculated based on the ratio of integration values at short intervals, then the coagulation time can be determined, but the measurement precision deteriorates due to fluctuations in scattered light amount
Solution Approach 1:
The patent divides the measurement period into multiple time intervals and calculates the average scattered light values across these intervals. This segmentation reduces the impact of short-term fluctuations in scattered light amount, thereby improving coagulation time measurement accuracy while maintaining reliability.
Solution Approach 2:
The patent performs preliminary smoothing and averaging operations on the scattered light data before calculating the coagulation time. This pre-processing stabilizes the scattered light amount values, reducing fluctuations and improving the accuracy of subsequent coagulation time determination.
3Measurement precision
If the saturation value is determined using a threshold value for the difference between input values, then the coagulation reaction endpoint can be identified, but the determination becomes difficult for samples with constant change in scattered light after coagulation
Solution Approach 1:
The patent performs preliminary smoothing processing on the scattered light data and calculates average values across multiple time intervals. This pre-processing creates a more stable baseline that makes it easier to identify the coagulation reaction endpoint even for samples with constant scattered light changes after coagulation, reducing determination difficulty.
Solution Approach 2:
The patent introduces average scattered light values calculated from multiple intervals as an intermediary measure. This intermediary provides a more stable reference point for determining the coagulation reaction endpoint, making the determination process easier and more accurate for challenging samples.
Applied Scientific Principles
This section explains which scientific principles are used to turn an abstract innovation direction into a practical engineering solution.
Function Achieved in This Case
This approach allows for more accurate determination of coagulation time by stabilizing area ratios and reducing errors, even in samples with persistent optical changes post-coagulation, enhancing specificity and accuracy in coagulation time measurement.
Implementation Method 1
radiates light on a blood sample within a transparent container to which reagent has been added, and calculates the coagulation time based on the coagulation saturation value (coagulation reaction end point) from the change in the amount of scattered light over time
Data Source
AI summary
A sample analyzer optically measures reaction of a sample mixed with reagent, and obtains optical information therefrom; generates a reaction curve representing change in the optical information over time; determines a first area prior to an evaluation target time (t0) and a second area after the evaluation target time (t0) wherein the first and second areas are formed between a baseline which is parallel to the time axis and a reaction curve from a first time (t1) prior to the optional evaluation target time (t0) to a second time (t2) after the evaluation target time, and determines the reaction end point based on the first and second areas; and obtains a characteristic of a sample based on the determined reaction end point, is disclosed. a sample analyzing method is also disclosed.