Sample Chamber Planar Element for Non-Adherent Cell Localization
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Solution Overview
Problem
Existing sample chambers struggle to effectively observe and examine non-adherent molecules or cells, as they are easily flushed away during liquid interchange in microfluidic structures.
Innovation Solution
A sample chamber design featuring a cover plate and bottom plate with a recess, incorporating a planar element with predetermined holes to securely hold and localize molecules or cells, allowing for precise observation even during liquid interchange, using materials like mesh or electron microscope grids with adjustable parameters for optimal examination.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Quantity of substance
If liquid interchange is performed in microfluidic structures, then liquid flow and substance exchange are enabled, but non-adherent molecules or cells are flushed away and cannot be observed
Solution Approach 1:
The planar element divides the reservoir into multiple compartments defined by holes, each hole acting as a separate containment zone. This segmentation allows liquid to flow through the structure while molecules or cells are confined within individual holes, preventing them from being flushed away during liquid interchange.
Solution Approach 2:
The planar element with holes serves as an intermediary structure between the liquid flow and the molecules or cells. It mediates the interaction by allowing liquid to pass through while physically restraining molecules or cells within the holes, thus enabling liquid interchange without losing non-adherent samples.
2Measurement precision
If molecules or cells are held in holes of a planar element, then precise localization and observation are enabled, but the structure complexity increases
Solution Approach 1:
The planar element with holes is extracted as a separate, standalone component that can be independently manufactured and then integrated into the sample chamber. This extraction simplifies the overall manufacturing process while providing precise localization functionality through the predetermined hole arrangement.
Solution Approach 2:
The planar element utilizes a porous structure with predetermined holes to achieve precise localization of molecules or cells. The porous design with controlled aperture sizes and patterns provides exact positioning without requiring complex mechanical structures, thus maintaining simplicity while enhancing measurement precision.
Data Source
AI summary
The invention relates to a sample chamber with a cover plate and a bottom plate which is joined thereto in a fixed manner, wherein a recess is provided in the cover plate so that a reservoir is formed by the bottom plate, wherein a planar element with holes is provided in the reservoir, wherein the holes have a predetermined arrangement and/or size.