Biological Sample Container Film Holding to Prevent Floating Damage
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Solution Overview
Problem
Existing methods for holding biological samples in liquid reagents cause deformation or scratching, leading to pseudofluorescence and reduced precision in fluorescence observation.
Innovation Solution
A biological sample holding container with a container main body and an annular holding member that holds a film covering the sample, indirectly securing the sample without direct pressure, using a film with a reticulated structure for easy reagent injection and light transmission.
Engineering Contradictions & Design Principles
Engineering Contradiction Analysis
1Reliability
If a lid member is inserted into the container main body to prevent floating of the biological sample, then the floating prevention is improved, but the biological sample may be deformed due to excessive pressure
Solution Approach 1:
The patent introduces a film as an intermediary between the holding member and the biological sample. The film covers the sample and allows the holding member to hold the film rather than directly pressing the sample, thus preventing floating while avoiding deformation. This mediator approach resolves the contradiction by decoupling the holding function from direct contact with the sample.
Solution Approach 2:
The patent uses a flexible film to cover the biological sample. The film is thin enough to allow optical observation but flexible enough to conform to the sample shape and provide gentle holding pressure. This flexible film structure prevents floating while maintaining sample integrity, addressing both floating prevention and deformation avoidance.
2Reliability
If a tape is used to hold the biological sample, then the floating prevention is improved, but the holding force gradually declines in liquid reagent
Solution Approach 1:
The film serves as a durable intermediary that distributes holding force across the sample surface. Unlike tape that degrades in liquid, the film-membrane system is designed to maintain its structural integrity and holding capability throughout the observation period, ensuring consistent floating prevention.
Solution Approach 2:
The flexible film structure provides continuous, distributed support to the sample. The film's flexibility allows it to adapt to sample movement while maintaining contact, and its material properties ensure it does not degrade like adhesive tapes, thus maintaining holding force throughout the observation duration.
3Reliability
If a needle is used to hold the biological sample, then the floating prevention is improved, but the sample is scratched
Solution Approach 1:
The patent employs a flexible film instead of rigid needles to hold the sample. The film's soft, conforming nature allows it to prevent floating through distributed pressure rather than concentrated points, completely avoiding scratching while maintaining effective holding.
4Manufacturing precision
If the holding member is made to fit tightly inside the side surface portion, then the holding precision is improved, but the film insertion becomes difficult
Solution Approach 1:
The film is inserted into the container main body before the holding member is installed. This preliminary action allows the film to be positioned correctly on the sample without the constraint of the holding member, after which the holding member is added to secure the film. This sequence resolves the contradiction by allowing tight fitting for precision while maintaining easy film insertion.
Data Source
AI summary
A biological sample holding container includes: a container main body having a bottom surface portion having a placement region for a biological sample and a side surface portion provided on the bottom surface portion so as to surround the placement region; and an annular holding member insertable and removable inside the side surface portion. The holding member holds a film covering the biological sample on the placement region with the bottom surface portion by being inserted inside the side surface portion together with the film.